Numb与P53蛋白在上皮性卵巢癌组织中的表达及临床意义

徐敏; 贺红菊; 张欢等

摘要: 目的探讨Numb与P53蛋白在上皮性卵巢癌中的表达、相关性及临床意义。HTH方法采用免疫组化PV二步法联合检测Numb与P53蛋白在20例正常卵巢组织、29例良性卵巢肿瘤组织、35例交界性卵巢肿瘤组织及87例上皮性卵巢癌组织中的表达，分析它们的临床意义。结果 Numb蛋白在正常卵巢组织、良性卵巢肿瘤组织、交界性卵巢肿瘤组织及上皮性卵巢癌组织中的阳性表达率分别为5%、13.8%、51.4%、70.1%，呈逐渐升高的趋势（ P<0.05）；不同卵巢癌分期及有无淋巴结转移的上皮性卵巢癌患者Numb蛋白表达率差异有统计学意义（ P<0.05），而不同年龄、病理类型、分化程度的患者间差异无统计学意义（ P>0.05）。P53蛋白在正常卵巢组织、良性卵巢肿瘤组织、交界性卵巢肿瘤组织及上皮性卵巢癌组织中的阳性表达率分别为0%、3.4%、28.6%、63.2%，呈逐渐升高的趋势（ P<0.05）；不同病理类型、分化程度、卵巢癌分期及有无淋巴结转移的上皮性卵巢癌患者P53蛋白表达率差异有统计学意义（ P<0.05），而不同年龄间表达率差异无统计学意义（ P>0.05）。Numb与P53蛋白在交界性卵巢肿瘤组织和上皮性卵巢癌组织中的表达均呈正相关（ r=0.488， P<0.05； r=0.231， P<0.05）。结论通过联合检测Numb与P53蛋白可能有助于上皮性卵巢癌的早期诊断及预后判断，两种蛋白可能共同参与上皮性卵巢癌的发生发展过程。

Abstract: Objective To investigate the expression and clinical significance of Numb and P53 proteins in epithelial ovarian carcinoma. Methods We co-tested the expressions of Numb and P53 proteins in epithelial ovarian tissues by immunohistochemistry, including 20 cases of normal tissues, 29 cases of benign tumors, 35 cases of borderline ovarian tumors and 87 cases of epithelial ovarian carcinoma. Their clinical significance was analyzed. Results The positive expression rates of Numb protein in normal ovarian tissues, benign ovarian tumors, borderline ovarian tumors and epithelial ovarian carcinoma were 5%, 13.8%, 51.4% and 70.1%, respectively, and with the rising trend and significantly difference among four groups ( P<0.05). The expression of Numb protein had significant positive correlation with stage and lymph node metastasis ( P<0.05), while Numb expression had no correlation with age, pathological type or histological grade ( P>0.05). The positive expression rates of P53 protein in normal ovarian tissues, benign ovarian tumors, borderline ovarian tumors and epithelial ovarian carcinoma were 0%, 3.4%, 28.6% and 63.2%, respectively, which had a gradually rising trend with significantly difference among four groups ( P<0.05). The expression of P53 protein in borderline ovarian tumors was significantly higher than those in normal ovarian tissues and benign ovarian tumors ( P<0.05). The expression of P53 protein in epithelial ovarian carcinoma group was significantly higher than those in the rest three groups ( P<0.05). Statistical significance was found between the expression of P53 protein and histological type, differentiation, stage, lymph node metastasis ( P<0.05); But Numb expression had no correlation with age ( P>0.05). The expression of Numb protein was positive correlated with the expression of P53 protein in borderline ovarian tumors and epithelial ovarian carcinoma ( r=0.488, P<0.05; r=0.231, P<0.05). Conclusion Co-testing Numb and P53 protein might be helpful for the diagnosis and evaluation of prognosis of epithelial ovarian carcinoma and two proteins might work together in the development process of epithelial ovarian carcinoma.