Abstract: Objective To study the effect of high mobility group chromosomal protein N2 on inhibiting cervical cancer in nude mice. Methods Model of cervical cancer were established in nude mice. They were randomly divided into 4 groups including negative control group, HMGN2 group, cisplatin group and HMGN2 with cisplatin group. After 4 injections, the tumor size were calculated and tumor tissues were stained by haematoxylin eosin (HE) staining. Results Transplated tumor models were established successfully. The tumor sizes of negative control group 〔(0.38±0.12） cm₃〕 were significantly lower than those of HMGN2 group 〔(0.14±0.07）cm₃, cisplatin group 〔(0.11±0.06） cm₃〕 and HMGN2 combined with cisplatin group 〔(0.11±0.07） cm₃〕. No differences were detected in HMGN2 group, cisplatin group and HMGN2 with cisplatin group in tumor sizes. The tumor inhibition rates of HMGN2 group, cisplatin group and HMGN2 with cisplatin group were 0.62±0.18, 0.71±0.17 and 0.70±0.18, respectively. The necrosis area were smaller in negative control group than in other three groups by HE staining. Conclusion HMGN2 has a significant inhibitory effect on transplanted cervical cancer in nude mice.