Abstract: Objective To investigate the effect of He-Ne laser therapy on wound healing after nail surgery. Methods Patients who needed nail surgery were divided into treatment group (n=20) and control group (n=20) randomly.The He-Ne laser was applied to the wound after nail surgery in treatment group. The wound healing time, the inflammation duration, the pain rating, and the nail plate full-grown time were compared with control group. Results No significant differences in baseline of two groups were observed. The wound healing time was (16.00±2.51) d in control group and (11.35±1.73 ) d in treatment group,the inflammation duration was (9.10±2.10) d in control group and (7.20±1.44) d in treatment group (P<0.05), both above differences were significant (P<0.05).The nail plate full-grown time was (21.00±2.13) weeks in treatment group, whereas (21.40±1.05) weeks in control group (P＞0.05).The pain rating of the 2 groups was different from 25 h (after 3 times treatment)(P<0.05). Pain disappeared in 85.0% (17/20) patients of treatment group after 49 h (5 times treatment), and completely disappeared (20/20) after 54 h (6 times treatment),which were faster than those of control group at the same time points (pain disappeared only in 50.0% and 98.5% of patients respectively). Conclusion He-Ne laser therapy can help wound healing after nail surgery.