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长链非编码RNA HOTAIR mRNA在卵巢癌中的表达

崔玲, 谢晓砚, 王和, 陈新莲, 刘珊玲, 胡丽娜

崔玲, 谢晓砚, 王和, 等. 长链非编码RNA HOTAIR mRNA在卵巢癌中的表达[J]. 四川大学学报（医学版）, 2013, 44(1): 57-59.
引用本文: 崔玲, 谢晓砚, 王和, 等. 长链非编码RNA HOTAIR mRNA在卵巢癌中的表达[J]. 四川大学学报（医学版）, 2013, 44(1): 57-59.
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CUI Ling, XIE Xiao-yan, WANG He, et al. Expression of Long Non-coding RNA HOTAIR mRNA in Ovarian Cancer[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(1): 57-59.
Citation: CUI Ling, XIE Xiao-yan, WANG He, et al. Expression of Long Non-coding RNA HOTAIR mRNA in Ovarian Cancer[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(1): 57-59.
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栏目: 论著

长链非编码RNA HOTAIR mRNA在卵巢癌中的表达

  • 1. 四川大学华西第二医院 西部妇幼医学研究院 遗传实验室(成都610041);

  • 2. 四川大学华西第二医院 妇产科教研室(成都 610041);

  • 3. 妇儿疾病与出生缺陷教育部重点实验室(成都 610041);

  • 4. 四川大学华西第二医院 西部妇幼医学研究院 细胞与基因治疗实验室(成都 610041)

基金项目: 

高端人才和创新团队(PCSIRT)发展计划(No.IRT0935)资助

Expression of Long Non-coding RNA HOTAIR mRNA in Ovarian Cancer

  • 1. Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second Hospital, Sichuan University, Chengdu 610041, China;

  • 2. Department of Obstetric and Gynecologic, West China Second Hospital, Sichuan University, Chengdu 610041, China;

  • 3. Key Laboratory of Obstetric&Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu 610041, China;

  • 4. Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second Hospital, Sichuan University, Chengdu 610041, China

摘要

    摘要
  • 摘要:

      目的   探讨长链非编码RNA HOX转录反义RNA(HOTAIR)在卵巢癌组织与正常卵巢组织中的表达差异。   方法   对44例卵巢癌组织及14例正常卵巢组织样本采用实时荧光定量PCR(RT-PCR)方法检测长链非编码RNA HOTAIR mRNA的表达。   结果   卵巢癌组织中HOTAIR mRNA表达高于正常卵巢组织[(1.26±0.27) vs. (0.14±0.09)],差异有统计学意义(P<0.05),病理类型为低度分化及未分化的卵巢癌组织中HOTAIR mRNA表达高于中-低、中-高及高度分化组织[(1.65±0.41) vs. (0.39±0.14)],差异有统计学意义(P<0.05)。   结论   卵巢癌组织中HOTAIR的表达增高,且癌组织分化越低,HOTAIR表达越高。提示HOTAIR有可能作为发现卵巢癌,并判断其恶性程度的一个分子标志。

     

    Abstract:

      Objective   To investigate the long non-coding RNA HOTAIR (long non-coding HOX antisense intergenic RNA) by examining HOTAIR expression levels in ovarian cancer tissue and normal ovarian tissue.   Methods   The mRNA of long non-coding RNA HOTAIR expressions were detected by real-time fluorescence quantitative PCR in ovarian cancer (44) and normal ovary tissues (14).   Results   The expression of HOTAIR in ovarian cancer tissue was higher than that in normal ovarian tissue (1.26±0.27 vs. 0.14±0.09, P<0.05). The expression was statistically higher in poorly differentiated ovarian cancer than poorly-moderately, moderately-well, and well-differentiated ones (1.65±0.41 vs. 0.39±0.14, P<0.05).   Conclusion   RNA HOTAIR expressions were higher in ovarian cancer; it may play a role in ovarian cancer, and become a biomarker for malignant degree of ovarian cancer and may provide a novel therapeutic target for ovarian cancer.

     

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    • 参考文献(12)
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    Yang Z, Zhou L, Wu LM, et al. Overexpression of long non-coding RNA HOTAIR predicts tumor recurrence in hepatocellular carcinoma patients following liver transplantation. Ann Surg Oncol,2011;18(5):1243-1250.
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出版历程
  • 收稿日期:  2012-07-09
  • 修回日期:  2012-10-31
  • 发布日期:  2013-01-19

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