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4-氨基-1,8-萘二胺对三氧化二砷在肝癌细胞中的增敏作用研究
4-amino-1,8-naphthalimide on the Sensitive Effect of Arsenic Trioxide in Hepatocellular Carcinoma Cells
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摘要: 目的 探讨聚腺苷酸二磷酸核糖转移酶-1（PARP-1）抑制剂4-氨基-1，8-萘二胺（4-AN）对三氧化二砷（ATO）治疗肝癌的敏感性影响及其相关机制。方法 培养人肝癌细胞HepG2，分为单独ATO用药组和联合用药组（4-AN+ATO），采用MTT实验、群体倍增实验和克隆形成实验比较两组细胞存活、增殖情况；应用ELISA、单细胞凝胶电泳（彗星实验）及微核实验分别检测细胞内8-羟基鸟嘌呤（8-OH-dG）含量，DNA链断裂、修复以及染色体损伤情况。结果 当ATO浓度为2～10 μmol/L时，联合用药组细胞存活率和集落形成率低于ATO组（\P\P\P＜0.05）；彗星实验检测损伤修复结果显示，联合用药组细胞的DNA损伤修复效率低于ATO组（\P\P<0.05）。结论 PARP-1抑制剂4-AN能显著增强ATO杀伤肝癌细胞的敏感性，其机制与抑制肝癌细胞DNA损伤修复有关。Abstract: Objective To study the effect and mechanism of 4-amino-1,8-naphthalimide(4-AN) on the sensitive effect of arsenic trioxide (ATO) in hepatocellular carcinoma cells. Methods Hepatocellular carcinoma HepG2 cells were divided into two groups according to whether they were treated with 4-AN or not. Cell viability was evaluated by MTT assay, population doubling experiment and colony formation assay; genic mechanism was explored by 8-OH-dG assay, single cell gel electrophoresis (comet assay) and micronucleus test. Results At 2-10 μmol/L concentration of ATO, the cell viability and colony formation efficiency of the combination group (4-AN+ATO) were significantly lower than that of the ATO group (\P\P\P\P\P<0.05). Conclusion 4-AN can significantly increase the sensitivity of ATO in treatment with hepatocellular carcinoma cells and prevent DNA damage repair may be a primary mechanism for this effect.
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期刊类型引用(2)
1. 陈虹宇, 谷仕艳, 张遵真. 双氢青蒿素对三氧化二砷在肺腺癌细胞的敏感性研究. 现代预防医学. 2017(14): 2600-2604 . 
百度学术
2. 胡亚男, 谷仕艳, 张遵真. 阿司匹林增加人肝癌细胞对三氧化二砷敏感性的实验研究及机制探讨. 四川大学学报(医学版). 2016(02): 159-163 . 百度学术
其他类型引用(2)
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