Abstract: Objective To study the clinical symptoms and the condition of skin barrier of different facial dermatitis. Methods The patients with facial dermatitis were divided into 4 groups based on their diagnosis: hormone-dependent dermatitis (HDD) group, sensitive skin (SS) group, cosmetic intolerance (CI) group and normal control group. All patients received the evaluation on clinical symptoms with measuring scale, measurements of stratum corneum water content (SCWC), sebum, pH, trans-epidermal water loss (TEWL) and blood flow. The skin scales were collected for the measurements of the ratio of immature cornified envelope (CE) via immunofluorescence and the concentration of kallikrein 5 (KLK5) via enzyme-linked immunosorbent assay. Results There were total 146 patients included (HDD 38, SS 53,CI 15, control group 30), most of them had their symptoms evaluated as moderate to severe. Compared with the control,the disease groups had lower SCWC and sebum (P<0.05), higher pH, TEWL and blood flow (P<0.05), higher ratio of immature CE (P=0.011) and lower concentration of KLK5 (P=0.000); while these parameters among the disease groups did not show statistical significant difference. Conclusion There is no difference in the clinical symptoms and the condition of skin barrier between facial dermatitis with different inducements. The growth of CE may be inhibited and the concentration of KLK5 decreases in facial dermatitis.