Abstract: Objective To examine the pregnancy outcomes of rabbits being subjected to pulmonary ventilation perfusion imaging examinations. Methods Pregnant rabbits were randomly divided into two groups: control group and experimental (lung ventilation-perfusion scintigraphy) group. The pregnancy outcomes were measured using indicators of miscarriage, premature birth, and stillbirth, as well as malformations and developmental abnormalities of offspring over a three-month period. Results No significant differences in miscarriage, premature birth, and stillbirth were found between the two groups. No obvious deformity appearances in the offspring were observed. The two groups showed no statistically significant differences in fetal progeny-intrauterine growth and developmental indicators measured by body mass, head circumference, abdominal circumference and length. Conclusion Lung ventilation-perfusion scintigraphy examination has no effects on pregnant outcomes measured by miscarriage, premature birth, stillbirth, fetal teratogenicity and fetal growth.