Abstract: Objective To generate systemic expression human cellular glutathione peroxidase-1 (GPx-1) (198Leu) transgenic mice model in order to investigate the functional variants in GPx gene in oxidative stress-related diseases. Methods After linearization with Bam HⅠ and AccⅠ, the transgenic construct GPx-1 (198Leu) was microinjected into the zygotes of C57BL/6J mice to generate transgenic mice, whose genotype was detected by PCR with specific primers. The GPx gene expression profile was determined by Western blotting. Results 13 transgenic founder mice were successfully generated. Western blotting result showed that the protein expression level of 4 transgenic mice in hearts were higher than that of wild type mice. Conclusion Human GPx-1 P198L transgenic mice was successfully established. This kind of animal model is of significance for making further researches on oxidative stress-related diseases.