Abstract: Objective?To investigate whether pilose antler blood wine has the effects of estrogen-like activity and antioxidant in the ovariectomized (OVX) female rats. Methods?The rat model of oxidative stress was established by ovariectomy. The female rats were divided into 6 groups: normal control group, OVX group, base wine group, and 3 pilose antler blood wine treated OVX groups (low, medium and high dose of wine at a dose of 4.5 g/kg, 9.1 g/kg and 13.6 g/kg respectively). After the feeding of pilose antler blood wine (saline in control and OVX group) for 31 days, the serum estrodial level, serum superoxide dismutase (SOD), and the content of methane dicarboxylic aldehyde (MDA) were measured. Results?The one month treatment of daily drink of pilose antler blood wine could significantly promote the serum estrodial level in OVX rats (P<0.05). Either low or medium dose could inhibit the decrease of serum SOD activity and the increase of MDA content in OVX rats, and the inhibition effects were more obvious in low dose group compared with high dose group (P<0.05). Conclusion?The daily drink of pilose antler blood wine showed the effects on increasing estrodial level and antioxidation in OVX rats.