Abstract: ObjectiveTo observe the expression mode and modulation effects of lncRNA-Paupar in the process of bupivacaine induced neurotoxicity. MethodsDorsal root ganglion (DRG) neurons were cultured and neurotoxicity model was produced on it. And the expression of lncRNA-Paupar was evaluated by qRT-PCR. Lnc-Paupar specific siRNA-P and random control group siRNA-C were constructed, 200 nmol/L siRNA-P, siRNA-C were transfected into ganglion cells and the apoptosis rate of transfected cells were observed by TUNEL method. The JNK and phosphorylated JNK (p-JNK) protein expression were observed with Western blot, respectively. ResultsLncRNA-Paupar was significantly up-regulated during the process of bupivacaine induced neurotoxicity and siRNA mediated lncRNA-Paupar down-regulation protected DRG neurons cells from apoptosis. Both JNK and p-JNK protein were reduced in siRNA transfected cells exemplified by Western blot. ConclusionLncRNA-Paupar could induced neurotoxicity through JNK pathway.