Abstract: Objective To explore the cytotoxicity of concentrate growth factors (CGF) and the effects on the apoptosis, proliferation and differentiation of human dental pulp cells (hDPCs), which were closely correlated with future application of CGF in the treatment of dental pulpal and periapical diseases. Methods hDPCs were isolated from permanent teeth extracted for orthodontic purpose, and expanded in vitro. hDPCs were treated with CGF and mineral trioxide aggregate (MTA) respectively. The cell apoptosis, proliferation, cell cycle and ALP activity were analyzed after 1, 3 and 7 days. Results Compared with the MTA group, CGF significantly promoted cell proliferation, increased the proportion of S-phase cells and ALP activity on days 3 and 7 (P<0.01). Besides, hDPCs apoptotic rates decreased in CGF group. Conclusion CGF has a good ability to promote the proliferation of dental pulp cells, resist apoptosis and induce osteogenic/odontogenic differentiation in vitro.