消退素RvD1减轻雨蛙素联合LPS诱导的小鼠重症急性胰腺炎的实验研究

叶丹; 王凤英; 刘祚燕等

消退素RvD1减轻雨蛙素联合LPS诱导的小鼠重症急性胰腺炎的实验研究

Resolvin D1 Reduces Cerulein and Lipopolysaccharide-induced Severe Acute Pancreatitis in Mice Fracture Patients

摘要

摘要: 目的探讨消退素（RvD1）对雨蛙素联合脂多糖(LPS)诱导的小鼠重症急性胰腺炎（SAP）的治疗作用。方法雨蛙素联合LPS诱导小鼠重症急性胰腺炎模型，并设立生理盐水对照组。建模4 h后，对小鼠一次性腹腔注射RvD1(150 mg/kg)进行干预处理，所有小鼠在开始造模24 h后取血，处死小鼠取胰腺标本，HE染色观察病理改变及评分；ELISA检测小鼠血清淀粉酶、脂肪酶水平，液相芯片检测小鼠血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）质量浓度。结果雨蛙素联合LPS成功诱导小鼠SAP模型（胰腺组织炎症性改变，病理评分增加，淀粉酶、脂肪酶升高）；RvD1干预明显减轻SAP组小鼠胰腺病理改变，降低淀粉酶、脂肪酶的水平，降低血清TNF-α、IL-6质量浓度。结论RvD1能够减轻雨蛙素联合LPS诱导的小鼠SAP炎症程度。

Abstract: ObjectiveTo investigate the therapeutic effects of resolvin D1 (RvD1) on cerulein and lipopolysaccharide (LPS)-induced severe acute pancreatitis in mice．MethodsThe model of severe acute pancreatitis was induced by cerulein combined with LPS in mice. Mice were treated with RvD1 at a dose of 150 mg/kg for 4 h after the last injection of cerulein. Pathological changes and scores were assessed by HE staining,serum amylase and lipase levels were detected by ELISA,serum tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) levels were determined by Luminex Assay. ResultsCerulein combined with LPS successfully induced severe acute pancreatitis model in mice. RvD1 reduced the pathological changes of pancreas in severe acute pancreatitis mice,decreased the serum levels of amylase and lipase,as well as attenuated the serum levels of TNF-α and IL-6. ConclusionRvD1 can reduce the severity of severe acute pancreatitis induced by cerulein and LPS in mice．

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