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加味四逆散对身心应激模型大鼠胃组织GASR、空肠组织VIPR2含量及其基因表达的影响

Effects of Jiaweisinisan Dispersion on Content and Gene Expression of Gastric Tissue GASR and Jejunal Tissue

    摘要
  • 摘要: 目的 观察加味四逆散对慢性身心应激胃溃疡模型大鼠胃黏膜超微结构、胃窦组织胃泌素受体（GASR）、空肠组织血管活性肠肽受体2（VIPR2）含量及其基因表达的影响，并阐明其机制。方法 将Wistar大鼠60只随机分为正常组，模型组，加味四逆散大、中、小剂量组，奥美拉唑组，每组10只。除正常组外，其余5组均采用慢性身心应激方法建立应激性胃溃疡大鼠模型。加味四逆散小、中、大剂量组大鼠每日分别灌胃0.25、0.5、1.0 g/mL浓度的中药煎剂2 mL，奥美拉唑组大鼠每日灌胃0.3 mg/mL的奥美拉唑溶液2 mL，正常组及模型组每日灌胃生理盐水2 mL，造模结束后，透射电镜法观察腺胃区胃黏膜组织细胞及细胞间连接的超微结构改变，免疫组化法和Real time-PCR法检测胃窦组织细胞GASR、空肠组织细胞VIPR2蛋白含量及其基因表达的变化。结果 电镜观察显示正常组大鼠胃黏膜上皮细胞间连接紧凑，胞膜完整，胞核形态及大小正常；模型组大鼠胃黏膜细胞受损严重；其余治疗组均较模型组不同程度好转。使用加味四逆散及奥美拉唑治疗后，各组胃窦组织GASR蛋白和mRNA的表达均较模型组增加(P<0.05)，空肠组织VIPR2蛋白和mRNA的表达均较模型组降低(P<0.05)。其中加味四逆散大剂量组GASR、VIPR2蛋白和mRNA的表达接近正常组水平，两者相比差异无统计学意义(P>0.05)。与奥美拉唑组与加味四逆散小剂量组相比，加味四逆散大剂量组大鼠GASR蛋白和mRNA的表达均增高(P<0.05)，VIPR2蛋白和mRNA的表达降低(P<0.05)。结论 加味四逆散可改善慢性身心应激胃溃疡模型大鼠胃黏膜组织细胞的微观病理形态，并可调整胃组织GASR和空肠组织VIPR2的含量及其基因表达。

     

    Abstract: Objective To observe the effects of Jiaweisini dispersion (JWSNS) on the ultrastructure of gastric mucosa, the content and gene expression of gastric antrum tissue gastrin receptor (GASR) and jejunal tissue vasoactive intestinal peptide receptor 2 (VIPR2) in chronic stress gastric ulcer rats, and to elucidate its mechanism. Methods 60 Wistar rats were randomly divided into normal group, model group, JWSNS large, medium, small dose groups, and omeprazole group, 10 rats in each group. Chronic stress method was used to establish the stress ulcer rat model. The every rat in JWSNS small, medium, large dose groups were gavaged with 0.25, 0.5, 1.0 g/mL Chinese medicine Decoction on 2 mL respectively daily, rats in omeprazole group were gavaged with 0.3 mg/mL omeprazole solution on 2 mL daily, rats in normal group and model group were gavaged 2 mL NS daily. After modeling was end, transmission electron microscopy (TEM) was used to observe gastric mucosa cells and intercellular connections changes of ultrastructure of glandular stomach area and immunohistochemical method and Real time-PCR method were used to detect the protein content and gene expression changes of gastric antrum tissue GASR and jejunal tissue cell VIPR2. Results TEM observation demonstrated that in the normal group the gastric mucosa epithelial cells connected compact, cell membrane integrity, cell nuclear shape and size was normal; in model group rats the gastric mucosal cells were severely damaged; the rats in the rest treatment groups were better than those in the model group in different degree. After The treatment of JWSNS and omeprazole, the expression of CM(155mmGASR protein and mRNA in gastric antrum tissue were increased when compared with that of model group (P<0.05), the expression of VIPR2 protein and mRNA in the jejunum tissue were lower than that of the model group (P<0.05). The expression of GASR, VIPR2 protein and mRNA in the JWSNS large dose group was closed to the normal group with no significant difference (P>0.05). And compared with omeprazole group and JWSNS small dose group, expression of GASR protein and mRNA in high dose group rats were increased (P<0.05), and expression of VIPR2 protein and mRNA were decreased (P<0.05). Conclusion JWSNS can significantly improve microscopic pathologic morphology of the gastric mucosa cell in gastric ulcer of chronic stress rats models, and can through two aspects of inhibiting damage factor and enhancing defense factor to adjust the content and gene expression of gastric tissue GASR and jejunal tissue VIPR2. 【Key words】

     

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