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不同剂量重组人促红细胞生成素在宫内缺血缺氧环境中透过胎盘屏障及血脑屏障的通透性

马玉姗, 周俊, 柳慧, 杜宇, 林雪梅

马玉姗, 周俊, 柳慧, 等. 不同剂量重组人促红细胞生成素在宫内缺血缺氧环境中透过胎盘屏障及血脑屏障的通透性[J]. 四川大学学报（医学版）, 2012, 43(5): 687-689,724.
引用本文: 马玉姗, 周俊, 柳慧, 等. 不同剂量重组人促红细胞生成素在宫内缺血缺氧环境中透过胎盘屏障及血脑屏障的通透性[J]. 四川大学学报（医学版）, 2012, 43(5): 687-689,724.
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MA Yu-shan, ZHOU Jun, LIU Hui, et al. Erythropoietin through the Placenta Barrier and Fetal Blood-Brain Barrier with Transient Uteroplacental Ischemia[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(5): 687-689,724.
Citation: MA Yu-shan, ZHOU Jun, LIU Hui, et al. Erythropoietin through the Placenta Barrier and Fetal Blood-Brain Barrier with Transient Uteroplacental Ischemia[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(5): 687-689,724.
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栏目: 论著

不同剂量重组人促红细胞生成素在宫内缺血缺氧环境中透过胎盘屏障及血脑屏障的通透性

  • 1. 四川大学华西第二医院 麻醉科 (成都 610041);

  • 2. 四川大学华西口腔医院 麻醉科 (成都 610041)

基金项目: 

四川省科技厅项目（No.2008JY0028-1）资助

Erythropoietin through the Placenta Barrier and Fetal Blood-Brain Barrier with Transient Uteroplacental Ischemia

  • 1. Department of Anesthesia, West China Second Hospital, Sichuan University, Chengdu 610041, China;

  • 2. Department of Anesthesia, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China

摘要

    摘要
  • 摘要: 目的 探讨不同剂量重组人促红细胞生成素（recombinant human erythropoietin，rhEPO）在宫内缺血缺氧时透过胎盘屏障及胎鼠血脑屏障的通透性。 方法 孕19 d（孕晚期）SD大鼠，分为3组：rhEPO治疗组（Treat组）、假手术对照组（Sham组）和生理盐水缺血对照组（I/R组）。Treat组和I/R组制备宫内缺血缺氧模型，Sham组只进行开关腹手术。Treat组和Sham组根据注射rhEPO的剂量不同各自再分为3组，分别在宫内缺血缺氧处理或开关腹手术前30 min经孕鼠尾静脉注射125I标记的rhEPO 2500 U/kg、5000 U/kg、7500 U/kg，I/R组在宫内缺血缺氧处理前30 min经尾静脉注入生理盐水。均于缺血缺氧处理或开关腹手术后24 h取胎盘、羊水及胎鼠脑、肝、心、肺和肾，检测并比较各组织中125I-rhEPO的放射比活性。 结果 Treat组和Sham组胎盘、羊水及胎鼠各组织中均能检测到125I-rhEPO的分布。125I-rhEPO在胎盘、羊水及胎鼠各组织中的分布均随rhEPO注射剂量的增加而增加。在各剂量组中，Treat组胎鼠各组织中的125I-rhEPO含量均高于Sham组（P<0.05）。 结论 在宫内缺血缺氧环境下，胎盘屏障及胎鼠血脑屏障对外源性rhEPO的通透性增加。

     

    Abstract: Objective To observe the permeability of recombinant human erythropoietin through placenta barrier and fetal blood-brain barrier after transient uteroplacental ischemia. Methods Rats on days 19 of pregnancy were divided into rhEPO treated group, ischemia-reperfusion group and sham-operated group. Fetal ischemia in rhEPO treated group and ischemia-reperfusion group was induced by bilateral occlusion of the utero-ovarian artery for 20 minutes. Different dosage of 125I-rhEPO (2500 U/kg,5000 U/kg,7500 U/kg) was injected into the rats through caudal veins 30 min before injury in rhEPO treated group and sham-operated group. Saline was administered intravenously 30 min before the induction of hypoxic-ischemic injury in ischemia-reperfusion group. The amniotic fluid, placenta and fetal organs including brain, liver, heart, lung and kidney were collected to measure the radioactivity at 24h after injury. Results 125I-rhEPO was detected in amniotic fluid, placenta and fetal organs. The radioactivity of 125I-rhEPO in these tissues increased gradually with the increased dose injected in rhEPO treated group and sham-operated group. There were significant differences in the radioactivity of 125I-rhEPO between rhEPO treated group and sham-operated group (P<0.05). Conclusion The permeability of rhEPO through placental barrier and blood-brain barrier increased under the condition of fetal ischemia and hypoxia.

     

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    • 参考文献(11)
  • [1]

    Verklan MT. The chilling details:hypoxic-ischemic encephalopathy. J Perinat Neonatal Nurs,2009;23(1):59-68.
    [2]

    Brines ML, Ghezzi P, Keenan S, et al. Erythropoietin crosses the blood-brain barrier to protect against experimental brain injury. Proc Natl Acad Sci USA,2000;97(19):10526-10531.
    [3] 周 俊, 马玉姗, 林雪梅等. 外源性重组人促红细胞生成素在宫内缺血缺氧环境中对胎盘屏障通透性的实验研究. 四川大学学报(医学版),2010;41(3):445-447.
    [4]

    Tanaka M, Natori M, Ishimoto H, et al. Experimental growth retardation produced by transient period of uteroplacental ischemia in pregnant Sprague-Dawley rats. Am J Obstet Gynecol,1994;171(5):1231-1234.
    [5]

    Koury MJ, Bondurant MC. The molecular mechanism of erythropoietin action. Eur J Biochem,1992;210(3):649-663.
    [6]

    Chong ZZ, Lin SH, Kang JQ, et al. Erythropoietin prevent early and late neuronal demise through modulation of Akt1 and induction of caspase 1, 3, and 8. J Neurosci Res,2003;71(5):659-669.
    [7]

    Sola A, Wen TC, Hamrick SE, et al. Potential for protection and repair following injury to the developing brain:a role for erythropoietin. Pediatr Res,2005;57(5):110-117.
    [8] 胡晓红, 栾 佐, 屈素清等. 碱性成纤维细胞生长因子对胎盘屏障通透性的实验研究. 第二军医大学学报,2001;22(7):677-679.
    [9]

    Banks WA, Jumbe NL, Farrell CL, et al. Passage of erythropoietic agents across the blood-brain barrier:a comparison of human and murine erythropoietin and the analog darbepoetin alfa. Eur J Pharmacol,2004;505(1-3):93-101.
    [10]

    Kaur C, Ling EA. Blood brain barrier in hypoxic-ischemic conditions. Curr Neurovasc Res,2008;5(1):71-81.
    [11]

    Statler PA, McPherson RJ, Bauer LA, et al. Pharmacokinetics of high-dose recombinant erythropoietin in plasma and brain of neonatal rats. Pediatr Res,2007;61(6):671-675.
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出版历程
  • 收稿日期:  2012-03-20
  • 修回日期:  2012-05-24
  • 发布日期:  2012-09-19

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