Abstract: Objective To investigate the effect of brucea javanica oil emulsion on the invasiveness of glioma cells and hosphatidylinositol 3 kinase/protein kinase B（PI3K/AKT) signaling pathway. Methods C6 glioma cells were treated by brucea javanica oil emulsion. The inhibition rate of glioma cells was detected by MTT, Western blot was used to detect protein expression levels of PI3K, AKT, and nuclear factor (NF)-κB in glioma cells. The number of the glioma cells migrated through polycarbonate membrane was detected by crystal violet staining method. Results Brucea javanica oil emulsion inhibited PI3K, AKT, and NF-κB protein expression which reached the highest inhibition at 30 min, 60 min, and 120 min after brucea javanica oil emulsion, respectively. Maximum suppression on the proliferation of C6 glioma cells reached at 180 min after brucea javanica oil emulsion, while the number of glioma cells migrated through polycarbonate membrane was the least. Conclusion Brucea javanica oil emulsion inhibit the proliferation and invasiveness of glioma cells, which may be related to the inhibition of PI3K/AKT signal pathway.