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DUOX2在银屑病及特应性皮炎皮损表达的研究
Expression of DUOX2 in Psoriasis and Atopic Dermatitis Lesion
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摘要: 目的 探讨双功能氧化酶(dual oxidase,DUOX)2在寻常型银屑病患者皮肤、特应性皮炎(atopic dermatitis,AD)患者皮肤及正常人皮肤中的表达定位及差异,探讨其在皮肤抗感染机制中的作用。 方法 免疫组化法检测银屑病皮损区、银屑病非皮损区、AD皮损区、AD非皮损区皮肤及正常皮肤DUOX2蛋白的表达。以逆转录聚合酶链反应(RT-PCR)法检测寻常型银屑病患者皮损区、AD皮损区及正常皮肤中DUOX2 mRNA表达水平。 结果 免疫组化法检测结果显示,DUOX2蛋白在银屑病皮损区、银屑病非皮损区、AD皮损区、AD非皮损区皮肤及正常皮肤标本中均有表达,并主要分布在表皮基底层、棘层与真皮乳头层。银屑病皮损组较银屑病非皮损组及正常皮肤组表达增加(P<0.01);AD皮损组较AD非皮损组及正常皮肤组表达增加(P<0.01);AD皮损组较银屑病皮损组表达增加(P<0.01)。RT-PCR法检测到DUOX2 mRNA在银屑病皮损及AD皮损中有表达,但差异无统计学意义(P>0.05)。 结论 DUOX2在寻常型银屑病皮损、AD皮损及正常皮肤中表达,提示DUOX2可能参与皮肤抗感染机制,发挥抗菌屏障功能及氧化防御作用。Abstract: Objective To investigate the expression of dual oxidase 2 (DUOX2) in psoriasis vulgaris lesions, atopic dermatitis (AD) lesions and normal skin and its role in cutaneous anti-inflammation. Methods Tissue samples were harvested from psoriasis lesion area, psoriasis non-lesion area, AD lesion area and AD non-lesion area, as well as normal skin, the expression level of DUOX2 protein was detected by immunohistochemical staining. The mRNA level of DUOX2 was detected by reverse transcription polymerase chain reaction (RT-PCR) analysis. Results The expression of DUOX2 protein was observed in all groups which mainly located in basal layer, spinous layer and dermal papilla layer. Compared with the psoriasis non-lesion group and normal skin group, the expression level of DUOX2 protein in psoriasis lesion group was significant higher (P<0.01). The expression of DUOX2 protein in AD lesion group was stronger than that in AD non-lesion group and normal skin group (P<0.01). In addition, the expression level of DUOX2 protein in AD lesion group was significant higher than that in psoriasis lesion group (P<0.01). RT-PCR test revealed DUOX2 mRNA was expressed positively in psoriasis and AD lesions. Conclusion The strong expression of DUOX2 in psoriasis vulgaris lesion and AD lesion suggested that DUOX2 may play an important role in the mechanisms of cutaneous anti-inflammation.
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Keywords:
- Psoriasis /
- Atopic dermatitis /
- Dual oxidase 2 /
- Immunohistochemistry /
- RT-PCR
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