Abstract: Objective? To investigate the effects of whole body vibration therapy (WBVT) on structural and functional remodeling of subchondral bone in rabbits with early Osteoarthritis (OA). Methods? Twenty-four adult rabbits received anterior cruciate ligament transaction (ACLT) to establish knee osteoarthritis model. The rabbits were randomly divided into two groups: ACLT group and WBVT+ACLT group. The rabbits in the WBVT+ACLT group received whole body vibration treatment (Frequency:40 Hz;Amplitude: 2-4 mm;40 min/d，5 d/week，4 weeks) two months after surgery. After interventions, the left femur and tibia of all rabbits were placed in a Micro-CT scanning system. The bone volume fraction (BVF), trabecular thickness (Tb.Th), trabecular spacing (Tb.Sp), trabecular number (Tb.N), volumetric BMD (vBMD), and tissue BMD (tBMD) of the bones of femoral condyle and tibia were analysed using three-dimensional reconstruction Micview V2.1.2 and ABA analysis software. Geomagic Studio 11.0 software were used to calculate modulus (EM), reaction force (RF) and average Von Miss stress (VMF). Results? Higher levels of BVF, Tb.N, Tb.Th, EM, RF, VMF, vBMD and tBMD and lower levels of Tb.Sp were found in distal femora and tibia of the rabbits in the WBVT+ACLT group compared with the controls (P＜0.05). Conclusion? WBVT can effectively improve bone microstructure and mechanical properties of rabbits with early knee osteoarthritis.