欢迎来到《四川大学学报(医学版)》 2025年4月3日 星期四

结直肠癌中K-ras基因突变与TAK1、MAP4K2蛋白表达的相关性研究

马其钊, 杨以文, 马岩林等

马其钊, 杨以文, 马岩林等. 结直肠癌中K-ras基因突变与TAK1、MAP4K2蛋白表达的相关性研究[J]. 四川大学学报(医学版), 2019, 50(1): 61-65.
引用本文: 马其钊, 杨以文, 马岩林等. 结直肠癌中K-ras基因突变与TAK1、MAP4K2蛋白表达的相关性研究[J]. 四川大学学报(医学版), 2019, 50(1): 61-65.
MA Qi-zhao, YANG Yi-wen, MAYan-lin. et al. Correlation of K-ras Gene Mutations with the Protein Expressions of TAK1 and MAP4K2 in Colorectal Cancer[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(1): 61-65.
Citation: MA Qi-zhao, YANG Yi-wen, MAYan-lin. et al. Correlation of K-ras Gene Mutations with the Protein Expressions of TAK1 and MAP4K2 in Colorectal Cancer[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(1): 61-65.

栏目: 论著

结直肠癌中K-ras基因突变与TAK1、MAP4K2蛋白表达的相关性研究

Correlation of K-ras Gene Mutations with the Protein Expressions of TAK1 and MAP4K2 in Colorectal Cancer

  • 摘要: 目的 分析K-ras基因突变、转化生长因子激酶1(TAK1)蛋白和MAP4K2蛋白的表达与结直肠癌临床病理特征的关系,并分析K-ras基因突变与TAK1、MAP4K2蛋白表达的相关性。方法 应用DNA测序法检测76例结直肠癌样本中K-ras基因突变情况,应用免疫组化法检测样本中TAK1和MAP4K2蛋白表达情况。结果 76例结直肠癌中,K-ras基因突变率为32.89%(25例),K-ras基因突变与肿瘤分化程度有关( P<0.05),与浸润深度、是否有淋巴结转移无关;TAK1阳性率为48.68%,MAP4K2阳性率为46.05%,TAK1蛋白表达和MAP4K2蛋白表达均与肿瘤分化程度、是否有淋巴结转移有关( P<0.05),与浸润深度无关。在76例结直肠癌中,K-ras基因突变与TAK1蛋白表达、MAP4K2蛋白表达均无相关性( P>0.05),TAK1蛋白表达与MAP4K2蛋白表达之间无相关性; 在25例K-ras基因突变的结直肠癌中,TAK1蛋白表达与MAP4K2蛋白表达之间呈正相关(r=0.402, P<0.028)。结论 K-ras基因突变、TAK1和MAP4K2蛋白表达均与结直肠癌的肿瘤分化程度有关,与浸润深度无关;在K-ras基因突变的结直肠癌中,TAK1蛋白表达与MAP4K2蛋白表达之间呈正相关。

     

    Abstract: Objective To analyze the correlation of K-ras gene mutations with the protein expressions of transforming growth factor-β activating kinase 1 (TAK1) protein and mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2) protein in colorectal cancer. Methods K-ras gene mutations were detected by DNA sequencing analysis, and the expressions of TAK1 protein and MAP4K2 protein were detected by immunohistochemical method in 76 cases of colorectal cancer tissues. Results In 76 cases of colorectal cancer tissues, the mutation rate of K-ras gene was 32.89% (25 cases), and K-ras gene mutations were correlated with the degrees of cell differentiation ( P<0.05). The positive rates of TAK1 protein and MAP4K2 protein were 48.68% and 46.05%, respectively. The protein expressions of TAK1 and MAP4K2 were positively correlated with the degrees of cell differentiation and lymph node metastases, respectively ( P<0.05). There was no correlation between K-ras gene mutation and either TAK1 protein or MAP4K2 protein expression ( P>0.05). In 25 cases of colorectal cancer with K-ras mutation, the expression of TAK1 protein was positively correlated with the expression of MAP4K2 protein ( P<0.05). Conclusion K-ras gene mutation, TAK1 and MAP4K2 protein expressions were related to the degree of differentiation of colorectal cancer, but not to the depth of invasion. In colorectal cancer with K-ras gene mutation, the expression of TAK1 protein was positively correlated with the expression of MAP4K2 protein.

     

  • 期刊类型引用(8)

    1. 王宁宁,刘娅静,尹仁龙,李静静. 脑瘫患儿粗大运动功能分级与高危因素的相关分析及预测模型的构建. 中国妇幼保健. 2022(14): 2596-2600 . 百度学术
    2. 王军,吴猛,周丽杰,郭倩玉,李兵兵,苏春娅,朱登纳. 脑瘫儿童高危因素及其与粗大运动功能的关联分析. 河南预防医学杂志. 2022(11): 801-805+829 . 百度学术
    3. 秦广莲. MRI常规扫描及SWI、DWI序列在新生儿缺氧缺血性脑病诊断中的应用价值研究. 四川解剖学杂志. 2021(01): 171-172 . 百度学术
    4. 易爽,余波,庞侯旭. 磷酸肌酸钠联合神经节苷脂治疗新生儿缺氧缺血性脑病的临床疗效. 川北医学院学报. 2021(05): 588-591 . 百度学术
    5. 齐倩,段晓娜. 扭动运动对早产儿脑瘫的早期预测效果分析. 中外医疗. 2020(15): 41-43 . 百度学术
    6. 马丹,王秋,罗蓉,赵金桂,王芳芳,李娜. 成都地区脑性瘫痪患儿422例伴发小头畸形临床分析. 中国实用儿科杂志. 2019(01): 46-49 . 百度学术
    7. 陈海. 康复综合治疗措施对痉挛型脑瘫儿童的运动功能恢复的效果及预后的影响分析. 数理医药学杂志. 2018(07): 1098-1099 . 百度学术
    8. 王玎. 脑性瘫痪儿童粗大运动功能分级与孕龄及出生体质量的关系. 中国妇幼保健. 2017(22): 5655-5657 . 百度学术

    其他类型引用(4)

计量
  • 文章访问数: 
  • HTML全文浏览量: 
  • PDF下载量: 
  • 被引次数: 12
出版历程
  • 发布日期:  2019-01-19

目录

    /

    返回文章
    返回