Abstract: Objective To compare the efficacy and safety between tension-free vaginal tape obturator technique (TVT-O) and TVT-O with biofeedback pelvic floor electrical stimulation on the therapy of female stress urinary incontinence. Methods In this prospective study, 120 female patients of female stress urinary incontinence were enrolled from January 2012 to December 2013. The patients were randomly assigned to two groups，60 in group A received TVT-O alone, while 60 in group B not only received TVT-O but also received biofeedback pelvic floor electrical stimulation. All the patients were followed up for 12 months to assess the efficacy and safety of the two procedures. Subjective indices 〔total volume of urine (TV) ,the total frequency of urination (TOV),the total leakage of urine events (TL), urinary incontinence related quality of life questionnaire (I-QOL),International Advisory Committee on urinary incontinence urinary incontinence questionnaire short form scale (ICI-Q-SF)〕 and objective indices 〔Valsalva leak point pressure (VLPP), maximum flow rate (MFR), residual urine volume (RUV),pad test〕 were analyzed. Results Overall, the cure rate was 75% at 1 year follow-up for group A and 88.33% for group B (P <0.05). The effective rate was 16.67% for group A and 10% for group B (P <0.05). The ineffective rate was 8.33% for group A and 1.67% for group B (P <0.05). TV,I-QOL, ICI-Q-SF, VLPP and pad test in group B were superior to group A （P <0.05）. Conclusion Pelvic floor electrical stimulation biofeedback therapy could improve the curative effects of TVT-O on the treatment of female stress urinary incontinence.