首页
血小板微粒及其表面标记物CD62P、CD154与 类风湿关节炎的相关性研究
Association of Elevated Platelet Microparticles with Disease Activity in Rheumatoid Arthritis
-
摘要: 目的观察血小板微粒（platelets microparticles, PMPs）在类风湿关节炎（RA）患者关节液和外周血中的表达及其与健康人外周血对照之间的差异，探讨PMPs水平与RA活动指标之间的关系。方法采用流式细胞术检测26例活动期〔疾病活动指数（DAS）28≥3.2〕RA患者外周血和15例健康对照组外周血PMPs含量、PMPs的CD62P 、CD154阳性比率，检测RA患者和15例健康对照组外周血C反应蛋白（CRP）、血沉（ESR）、类风湿因子（RF）、抗环瓜氨酸肽抗体（ACPA）指标，分析PMPs含量与各指标相关性。其中16例有膝关节积液RA患者同时检测配对外周血和关节液PMPs含量及CD62P+PMPs和CD154+PMPs比率。结果①RA患者外周血PMPs含量高于健康对照组（P<0.01），RA患者外周血PMPs含量高于配对关节液（P<0.01）； ②RA患者外周血CD62P+PMPs比率高于健康对照组（P<0.05）和配对关节液（P<0.05）；③RA患者外周血CD154+PMPs比率高于健康对照组（P<0.01）和配对关节液（P<0.05）；④RA患者外周血PMPs含量与疾病活动性评分DAS28 ( r=0.462, P=0.018)呈正相关,与ESR、CRP、RF、ACPA不存在相关性；而关节液PMPs含量与CRP、ESR、RF、ACPA、DAS28均无相关性。结论RA患者外周血PMPs表达异常，且与疾病活动性有关，提示PMPs在RA疾病活动和关节损害中发挥重要作用。Abstract: Objective To explore the expression of platelets microparticles (PMPs) in peripheral blood (PB) and synovial fluid (SF) of rheumatoid arthritis (RA) patients and its correlation with clinical inflammatory parameters. Methods The levels of PMPs in PB were detected by flow cytometry in 26 active RA patients and 15 healthy control (HC). SF was collected from 16 patients. The percentages of CD62P+PMPs, CD154+PMPs and clinical parameters (including CRP, ESR, RF and ACPA) were also measured, then the correlations of PMPs with these parameters were analyzed. Results PMPs levels in PB of RA patients were higher than those in PB from HC and those in SF of RA patients (P< 0.01). CD62P+PMPs levels in PB of RA patients were higher than those in PB of HC and those in SF of RA patients (P< 0.05). CD154+PMPs levels in PB of RA patients were higher than those in PB of HC (P< 0.01) and those in SF of RA patients (P< 0.05). The levels of PB PMPs were positively correlated with disease activity score DAS28 ( r=0.462, P=0.018), but not with ESR, CRP, RF or ACPA. The levels of SF PMPs were not correlated with any of them (P>0.05). Conclusion PMPs may be involved in immune regulation and systemic inflammation of RA. The elevated levels of PMPs could be a potential biomarker for RA.
-
-
期刊类型引用(6)
1. 纵瑞凯，刘健，章平衡，张晓军，王男，黄旦，文建庭，张瑞，陈唤明. 新风胶囊通过调节FAK/Calpain信号通路抑制佐剂性关节炎大鼠血小板活化. 时珍国医国药. 2022(08): 1814-1817 . 
百度学术
2. 胡志清，赵星鹏，刘坦. 血小板微粒表面CD62p在缺血性脑卒中中的检测价值. 河南医学研究. 2021(33): 6298-6301 . 百度学术
3. 郭方君，杨人强. 血小板微粒促进动脉粥样硬化发生的研究进展. 基础医学与临床. 2020(11): 1551-1555 . 百度学术
4. 刘莺燕. 血沉参数变化与类风湿关节炎患者病情活动的关系. 中国医学创新. 2019(21): 55-58 . 百度学术
5. 杜芹，刘向红，田鲲，孟姝. 类风湿性关节炎患者牙周状况及唾液微生物多样性分析. 四川大学学报(医学版). 2019(06): 935-940 . 百度学术
6. 刘磊，刘健，黄传兵，万磊，谌曦. 佐剂性关节炎大鼠血小板微粒、血小板参数及滑膜组织的变化. 中国临床保健杂志. 2018(05): 656-660 . 百度学术
其他类型引用(4)
计量
- 文章访问数: 3788
- HTML全文浏览量: 408
- PDF下载量: 44
- 被引次数: 10
下载:
