Abstract: Objective To analyze the difference of liver enzymes in different metabolism state groups of chronic hepatitis B (CHB). Methods We use prospective cross-sectional study to analyze the difference of liver enzymes in different metabolism state groups in 110 cases of CHB, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and glutamyl transferase (GGT). Results Regardless of the presence or absence of fatty liver, the levels of ALP and GGT were increased along with the deterioration of glucose metabolism (P<0.05).The levels of ALP and GGT in the presence of fatty liver group were higher than those in the absence of fatty liver group (P<0.05). The levels of AST, ALP and GGT showed the trend of increasing along with the increase of HOMA-IR and the decrease of HOMA-β. There was no difference of liver enzymes among the groups with or without other metabolism disorder (P>0.05). Conclusions In CHB, abnormal glucose metabolism and fatty liver can lead to the increase of ALP and GGT. The increase of HOMA-IR and the decrease of HOMA-β may lead to the increase of AST,ALP and GGT. Other metabolism disorder did not show any effect on the level of liver enzymes.