Abstract: With the continuous development of robot-assisted single-port laparoscopic surgery in gynecology, its indications have gradually expanded from benign gynecologic diseases to malignant tumors, reflecting an emerging trend toward the integration of robotic technology and single-port minimally invasive surgery. This review primarily discusses the clinical application of robotic systems such as the da Vinci Xi platform and domestically developed single-port robotic systems in various complex surgical procedures, as well as the development of technical strategies for optimizing instrument layout and overcoming operative limitations. From a technical perspective, the use of 3-dimensional high-definition visualization, flexible wristed instrumentation, and tremor filtration, combined with innovative surgical paradigms, has effectively addressed the problem of instrument interference in single-port surgery, thereby enabling precise resection, rapid postoperative recovery, and superior cosmetic outcomes with minimal visible scarring. Although dedicated single-port systems still face technical challenges and long-term outcome data remain limited, the future integration of artificial intelligence and remote technologies will further validate their clinical efficacy and promote the standardized application of this approach in gynecological clinical practice.