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Study on the Effect of Polystyrene-Polyvinylpyrrolidone Electrospun Fibre in Inhibiting the Adhesion of Porphyromonas gingivalis
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摘要:目的 探究聚苯乙烯（PS）及PS-聚乙烯吡咯烷酮（PVP）静电纺丝材料对牙龈卟啉单胞菌黏附能力的影响。方法 采用不锈钢针头及高压电场制备PS及PS-PVP静电纺丝材料；通过扫描电子显微镜观察不同材料表面的牙龈卟啉单胞菌生长黏附状态；通过菌落形成单位（colony forming unit, CFU）测定不同材料表面的牙龈卟啉单胞菌生物膜形成量的变化；通过改变静电纺丝材料表面的电荷性质测定材料表面电荷对牙龈卟啉单胞菌黏附能力的影响。结果 扫描电镜结果显示，PS与PS-PVP均可形成直径0.2 μm的静电纺丝纤维。通过对24 h及48 h的生物膜进行扫描电镜和CFU计数，发现在两种材料表面，牙龈卟啉单胞菌的生物膜黏附量均较少（P<0.05）。在四丁基溴化铵（tetrabutylammonium bromide, TBAB）处理后，PS-PVP静电纺丝材料的表面电荷由负电转为正电，且表面细菌黏附量上升，与未处理的PS及PS-PVP相比差异有统计学意义（P<0.05）。结论 PS及PS-PVP静电纺丝材料可有效降低牙龈卟啉单胞菌的黏附能力，且这种能力可能与材料表面电荷性质相关。Abstract:Objective To explore the effect of polystyrene (PS) and PS-polyvinylpyrrolidone (PVP) electrospun materials on the adhesion ability of Porphyromonas gingivalis (P. gingivalis), a common periodontal pathogen.Methods PS and PS-PVP electrospun materials were prepared with stainless steel needles in high-voltage electric field. The growth and adhesion of P. gingivalis on the surface of different materials were observed with scanning electron microscope (SEM). The changes in the amount of P. gingivalis biofilm formed on the surface of different materials were measured according to viable colony forming units (CFU). The effect of surface charge of the different materials on the adhesion ability of P. gingivalis was determined through changing the charge properties on the surface of the electrospun materials.Results SEM images showed that both PS and PS-PVP can be used to form electrospun fibers with a diameter of 0.2 μm. SEM images and CFU counts of the biofilm at 24 h and 48 h showed that there was a smaller amount of P. gingivalis biofilm on the surface of the two materials (P<0.05). After treatment with tetrabutylammonium bromide (TBAB), the surface charge of the PS-PVP electrospun material changed from being negatively charged to being positively charged, and the amount of bacterial adhesion on the surface increased significantly in comparison to that of untreated PS and PS-PVP materials (P<0.05).Conclusion PS and PS-PVP electrospun materials can be used to reduce the adhesion ability of P. gingivalis on the surface of different materials, and this ability may be related to the surface charge properties of the materials.
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Keywords:
- Porphyromonas gingivalis /
- Adhesion /
- Electrospun material /
- Surface potential
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图 1 两种不同材料静电纺丝膜的形态
Figure 1. The appearance of electrospun membranes of the two different materials
A: PS electronspun fibre; B: PS-PVP electronspun fibre.
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图 2 不同介质表面的生物膜黏附情况
Figure 2. Adhesion of biofilm on the surface of different materials
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图 3 三种不同介质表面的活菌菌落计数
Figure 3. Colony forming units on the surface of the three different materials
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