垂体腺瘤中干细胞样细胞的培养及初步鉴定

周培志; 马卫朝; 王修杰; 程书文; 姜曙

垂体腺瘤中干细胞样细胞的培养及初步鉴定

Isolation and Preliminary Identification of Stem-like Cells in Pituitary Adenoma

摘要

摘要: 目的检测垂体腺瘤组织中干细胞标志物的表达情况,培养并初步鉴定垂体瘤干细胞。方法提取42例液氮冻存的垂体腺瘤组织标本中的总RNA,通过RT-PCR方法检测瘤组织中Nestin的表达情况;通过免疫荧光法检测无血清条件下培养的垂体瘤细胞球中干细胞标志物(Nestin、Sox2)的表达情况。结果 42例肿瘤组织标本中,Nestin mRMA阳性表达37例;无血清培养条件富集培养的悬浮细胞球中均可通过免疫荧光法检测出Nestin、Sox2的表达。结论垂体腺瘤组织中存在干细胞标志物的表达,通过无血清培养亦可培养出垂体瘤干细胞。

Abstract: Objecitve To isolate and identify the pituitary adenoma stem-like cells from pituitary adenoma tissue. Methods Total RNA was prepared with 42 cases of pituitary adenoma tissue samples frozen in liquid nitrogen, the expression of Nestin was detected by RT-PCR. Tumor spheres were cultured in serum-free conditions and the immunefluorescence were used to detect the expression of stem cell markers such as Nestin, Sox2. Results The positive rate of Nestin mRNA expression was 88.1% (37/42). The cultured tumor spheres were found positive for Nestin, Sox2 by immunefluorescence detection. Conclusion Stem cell markers are expressed in the pituitary adenoma tissue and the pituitary adenoma stem-like cells can be cultured in serum-free condition.

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