真皮多潜能细胞注射对皮肤弹性的影响

薛斯亮; 李利; 黄珊珊等

真皮多潜能细胞注射对皮肤弹性的影响

Effect of Dermal Multipotent Cells Transplantation on Skin Elasticity in Aged Murine

摘要

摘要: 目的探讨真皮来源多潜能细胞（dermal multipotent cells，DMCs）注射对皮肤弹性的影响。方法从3 d 龄BALB/c小鼠真皮中分离培养获得DMCs，取第3代DMCs观察形态和成脂、成骨分化。将36只12月龄BALB/c小鼠分为DMCs组（n=12）、成纤维细胞（FBs）组（n=12）及对照组（n=12），分别将10⁴个DMCs、10⁴个FBs和100 μL DMEM培养基注射入小鼠双下肢之间背部中央部位的皮下作单点注射。于2周及4周使用MPA580皮肤弹性测试仪检测小鼠皮肤弹性变化。结果 DMCs接受诱导分化后，分别成脂、成骨。接受DMCs注射的小鼠皮肤弹性较对照组在2周后有较大增加（ P=0.000），但与FBs组差异无统计学意义（ P=0.216）。4周后皮肤弹性不仅较对照组增加（ P=0.000），且亦较FBs组增加（ P=0.031）。结论 DMCs注射在小鼠皮下可以增加皮肤的弹性。

Abstract: Objective To investigate the elasticity changes in aged dermis after injection of dermal multipotent cells (DMCs). Methods Dermal multipotent cells were isolated and cultured from 3-day-old BALB/c mice and then transplanted into the dermis of aged (12-week-old) BALB/c mice. Adult fibroblasts（FBs） were employed as control. At 2 and 4 weeks after the transplantation, we examined dermal elasticity by MPA580 skin test machine. Results The skin elasticity were improve at 2 weeks after the transplantion in both DMCs group and FBs group ( P= 0.000) but have no statistical difference between these two groups ( P=0.216). The different effect between these two groups appeared in 4 weeks after the transplantation ( P=0.031）. Conclusion Dermal multipotent cells appear more effective than fibroblasts in increasing skin elasticity.

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