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周琛云, 朱晓霞, 唐琳, 等. 多模态超声成像在判定超早期大鼠脑缺血模型成败的应用价值[J]. 四川大学学报（医学版）, 2019, 50(3): 344-349.
引用本文: 周琛云, 朱晓霞, 唐琳, 等. 多模态超声成像在判定超早期大鼠脑缺血模型成败的应用价值[J]. 四川大学学报（医学版）, 2019, 50(3): 344-349.
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ZHOU Chen-yun, ZHU Xiao-xia, TANG Lin, et al. Application of Multimodal Transcranial Ultrasound in SD Rats of Cerebral Ischemic Model at Super Early Stage[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(3): 344-349.
Citation: ZHOU Chen-yun, ZHU Xiao-xia, TANG Lin, et al. Application of Multimodal Transcranial Ultrasound in SD Rats of Cerebral Ischemic Model at Super Early Stage[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(3): 344-349.
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多模态超声成像在判定超早期大鼠脑缺血模型成败的应用价值

Application of Multimodal Transcranial Ultrasound in SD Rats of Cerebral Ischemic Model at Super Early Stage

    摘要
  • 摘要:
      目的  探讨多模态超声成像技术（mUS）在超早期（造模后5～15min）判定大鼠脑缺血模型成败的应用价值。
      方法  将磨薄局部颅骨后的SD大鼠15只进行线栓法局灶性脑缺血模型（MCAo）的制备。造模前、造模后即刻（超早期）行经颅灰阶超声、超声造影及增强彩色多普勒（CECDUS）超声成像，分别观察线栓在颅内的走行、右脑灌注成像及颅内大动脉的血流信号并进行测量。梗塞3 h后行大鼠改良神经功能缺损评分（mNSS）和脑组织2, 3, 5-氯化三苯基四氮唑（TTC）染色。以TTC染色联合mNSS评分判断造模成功为金标准，使用McNemar检验比较mUS和金标准两种方法阳性检出率是否有差异。
      结果  1只大鼠造模中死亡，共14只完成实验。mUS在造模后即刻判断造模成功的阳性检出率为71%（10/14），McNemar检验发现其阳性检出率与金标准（64%，9/14）间差异无统计学意义（P>0.05）。造模成功大鼠灰阶模式下可显示右侧脑底部双线状线栓强回声，超声造影模式下显示右脑实质内存在局灶性低灌注区，增强彩色多普勒模式下可见右侧大脑中动脉及大脑前动脉血流信号消失。分析造模成功大鼠右脑的时间强度曲线（TIC）发现，造模前与造模后右脑实质的峰值强度（PI）、曲线下面积（AUC）、增强斜率（WIS）、达峰时间（TTP）差异有统计学意义。
      结论  初步发现应用多模态超声成像技术可于超早期快速准确地判断大鼠MCAo造模是否成功。

     

    Abstract:
      Objective  To determine the value of applying multimodal ultrasound (mUS) in SD rats of cerebral ischemic model at super early stage (5-15 min after modeling).
      Methods  Fifteen focal cerebral ischemic models were established in SD rats with thinning skulls using the suture method. Gray-scale ultrasound, contrast-enhanced ultrasound, and enhanced color Doppler (CECDUS) were performed before and immediately after the modeling to observe the location of the in-cranial suture, perfusion of the right hemisphere, and color flow signal of the middle cerebral artery and the anterior cerebral artery, respectively.A modified neurological deficit score (mNSS) and 2, 3, 5-triphenyltetrazolium chloride (TTC) stains were obtained three hours later to confirm the successful modeling as the gold standard. The positive rate detected by mUS was compared with the gold standard using McNemar tests.
      Results  One rat died and 14 rats completed the experiment.mUS imaging detected 71% (10/14) positive signals, no significant difference compared with the gold standard (64%, 9/14) (P>0.05). A hyperechoic double-line at the bottom of the right brain and focal hypoperfused areas in the right hemisphere were observed by gray scale ultrasound and contrast-enhanced ultrasound in the successfully modeled rats, respectively. The CECDUS found no blood flow in the anterior and middle cerebral arteries. Time intensity curve (TIC) analyses indicated significant changes in peak intensity (PI), area under the curve (AUC), wash in slope (WIS), and time to peak (TTP) after successful modeling.
      Conclusion  Multimodal ultrasound can assess modeling success quickly and accurately immediately after the establishment of ischemic model of SD rats.

     

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