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刘美艳, 潘晓丽, 李学波, 等. 芦荟大黄素金属配合物的抗氧化活性研究[J]. 四川大学学报(医学版), 2021, 52(2): 241-247. DOI: 10.12182/20201160110
引用本文: 刘美艳, 潘晓丽, 李学波, 等. 芦荟大黄素金属配合物的抗氧化活性研究[J]. 四川大学学报(医学版), 2021, 52(2): 241-247. DOI: 10.12182/20201160110
LIU Mei-yan, PAN Xiao-li, LI Xue-bo, et al. Study on the Antioxidant Activity of Aloe-Emodin Metal Complex[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(2): 241-247. DOI: 10.12182/20201160110
Citation: LIU Mei-yan, PAN Xiao-li, LI Xue-bo, et al. Study on the Antioxidant Activity of Aloe-Emodin Metal Complex[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(2): 241-247. DOI: 10.12182/20201160110

芦荟大黄素金属配合物的抗氧化活性研究

Study on the Antioxidant Activity of Aloe-Emodin Metal Complex

  • 摘要:
      目的  合成3种芦荟大黄素金属配合物,并比较配体和配合物的抗氧化活性。
      方法  在无水乙醇溶剂中,通过溶解、搅拌合成芦荟大黄素-铁(Ⅱ)、芦荟大黄素-铜(Ⅱ)、芦荟大黄素-镁(Ⅱ)3种配合物,并对其结构进行表征,采用Fe2+-H2O2-亚甲蓝法、二苯代苦味肼基自由基法(DPPH法)等检测配体和配合物对超氧自由基(O2•)、羟基自由基(•OH)、二苯代苦味肼基自由基(DPPH•)的清除作用。
      结果  成功合成芦荟大黄素-铁(Ⅱ)、芦荟大黄素-铜(Ⅱ)、芦荟大黄素-镁(Ⅱ)3种配合物,根据结构表征结果,推测芦荟大黄素金属配合物可能结构为两分子芦荟大黄素通过9位羰基和8位羟基与一分子金属离子(Fe2+、Mg2+、Cu2+)形成配合物。配合物和配体对3种自由基均有清除作用,但配合物的清除作用强于其配体(P<0.05)。
      结论  芦荟大黄素与Fe2+、Cu2+、Mg2+ 3种金属离子配位后,增强了其配体本身的抗氧化活性。

     

    Abstract:
      Objective   To synthesize three kinds of metal complexes of aloe-emodin and compare the antioxidant activities of the ligands and the complexes.
      Methods  Three kinds of aloe emodin metal complex, the aloe-emodin-iron (Ⅱ), the aloe-emodin-copper (Ⅱ) and the aloe-emodin-magnesium (Ⅱ) complexes, were synthesized by dissolving and stirring in anhydrous ethanol solvent, and their structures were characterized. The Fe2+-H2O2-methylene blue method, the diphenyl bitter hydrazine radical method (DPPH method) and other assays were used to determine the clearance effect of ligands and complexes on superoxide radicals (O2•), hydroxyl radicals (•OH) and phenyl bitter hydrazine radical (DPPH•).
      Results  Three kinds of aloe emodin metal complex, the aloe-emodin-iron (Ⅱ), the aloe-emodin-copper (Ⅱ) and the aloe-emodin-magnesium (Ⅱ) complexes, were successfully synthesized. According to the results of structural characterization, we speculated that the aloe-emodin metal complexes were formed at the site between the two molecules of aloe-emodin and one molecule of metal ions (Fe2+, Mg2+, Cu2+) via the 9th carbonyl and 8th hydroxyl groups of the aloe-emodin molecules. Both the complex and the ligand have clearance effects on three kinds of free radicals, and the complex showed stronger effects than its ligand (P<0.05).
      Conclusion  Coordination of aloe-emodin with metal ions, such as Fe2+, Cu2+, and Mg2+, could enhance the antioxidant activity of the ligand itself.

     

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