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袁斗, 谭琛, 姚建民, 等. 耐力训练对兔心房颤动的影响及机制探索[J]. 四川大学学报（医学版）, 2020, 51(2): 213-218. DOI: 10.12182/20200360203
引用本文: 袁斗, 谭琛, 姚建民, 等. 耐力训练对兔心房颤动的影响及机制探索[J]. 四川大学学报（医学版）, 2020, 51(2): 213-218. DOI: 10.12182/20200360203
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YUAN Dou, TAN Chen, YAO Jian-min, et al. Effects of Continuous Training on the Induction of Atrial Fibrillation in a Rabbit Model[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(2): 213-218. DOI: 10.12182/20200360203
Citation: YUAN Dou, TAN Chen, YAO Jian-min, et al. Effects of Continuous Training on the Induction of Atrial Fibrillation in a Rabbit Model[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(2): 213-218. DOI: 10.12182/20200360203
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耐力训练对兔心房颤动的影响及机制探索

Effects of Continuous Training on the Induction of Atrial Fibrillation in a Rabbit Model

    摘要
  • 摘要:
      目的  通过耐力运动训练兔动物模型探索运动相关性心房颤动(AF)的发生机制。
      方法  36只新西兰大耳兔随机分为对照组、中强度耐力训练组、高强度耐力训练组（每组12只）：对照组不做任何耐力训练处理；耐力运动训练组每周训练5 d，每天1 h或一次性力竭（不足1 h），共持续16 周（中强度耐力训练组：坡度0度，速度15 m/min；高强度耐力训练组：坡度0度，速度30 m/min）。训练完成后，各组随机各取6只，共18只兔采用离体心脏Langendorff系统进行灌流，将1 μmol/L乙酰胆碱（Ach）+不同浓度的氯化钡（BaCl2）溶液（0、0.1、0.5、1、3、6 μmol/L）灌入冠脉内，开始持续给药过程中电生理指标的测定及记录。在左心耳置入的电极分别记录相应的心房有效不应期（AERP）及90%动作电位时程（APD90）、并给予心脏期前程序刺激（S1S2）诱发房颤，记录并计算房颤发生率。灌流完后剪取兔左心房，经实时荧光定量（qRT）-PCR 技术检测心房肌组织内向整流钾电流/通道（IK1）kir2.1、kir2.2、胶原蛋白Ⅰ和胶原蛋白Ⅲ的mRNA表达量。取各组剩余6只实验兔，共18只取左心房组织标本, 应用Masson 染色进行组织学检查，测定心肌组织胶原容积分数。
      结果  长期高强度的运动训练16周后，不同组间，Ach背景相同，BaCl2剂量相同作用下，随着运动强度的增加，APD90、AERP相应缩短, 房颤发生率增加，BaCl2 取0～6 μmol/L时，高强度耐力训练组与其余两组间比较差异均有统计学意义（P<0.001），而其余两组之间差异无统计学意义。同一组内，Ach背景相同，随着BaCl2剂量的增加，APD90、AERP相应延长，房颤发生率降低，3组组内比较，差异均有统计学意义（P<0.05）。随着运动量的增加，兔左心房kir2.1、kir2.2、胶原蛋白Ⅰ和胶原蛋白Ⅲ的 mRNA表达量均增加，胶原容积分数增加，高强度耐力训练组>中强度耐力训练组>对照组，差异有统计学意义（P<0.05）。
      结论  长期的高强度耐力运动训练可通过增加实验兔心房组织纤维化程度、心肌细胞IK1数量和（或）功能，改变心电传导，促使实验兔房颤发生和维持。

     

    Abstract:
      Objective  This study was aimed to assess mechanisms underlying continuous training induced atrial fibrillation (AF) in an animal model.
      Methods  Healthy New Zealand rabbits were divided into three groups (n=12 each): control group (C), moderate intensity group (M), and high intensity group (H). The intensity of continuous training was adjusted according to the treadmill speed. After 12 weeks of training, with a Langendorff perfusion system, AF was induced by S1S2 stimulation and the incidence was recorded. Changes in atrial kir2.1, kir2.2, type Ⅰ and Ⅲ collagen protein mRNA expressions were assessed by quantitative real-time PCR. Masson staining was used to assess the extracellular collagen volume fraction (CVF) .
      Results  After 12 weeks, comparing with group C, groups M and H had greater (P<0.05): CVF, incidence of AF (P<0.05, also between Groups H and M), and atrial inward rectifier potassium current/channel (IK1) . In Group H, kir2.1, kir2.2, type Ⅰ and Ⅲ collagen protein mRNA expressions in the left atrium were increased (P<0.05, compared with Groups C and M).
      Conclusion   Long-term and high-intensity treadmill running could increase AF incidence in rabbits.

     

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