Effects of Side-effect Attenuation Prescriptions on Cyclophosphamide-induced Myelosuppression in Mice

Objective To determine the effects of Side-effect Attenuation Prescriptions on the levels of white blood cells (WBC), hemoglobin (HGB) and platelet (PLT) in peripheral blood of mice influenced by cyclophosphamide (CTX). Methods Mice were randomly divided into 6 groups: normal control group, myelosuppression group induced by CTX (model group), recombinant human granulocyte colony stimulating factor (G-CSF) group, and Side-effect Attenuation Prescriptions group (experimental groups with high, middle, and low dosages). Marrow depressed models were established by injecting CTX intraperitoneally (40 mg/kg, once/d, 10 d) to the mice. High, middle, and low dosage experimental groups received 40 g/kg, 20 g/kg, and 10 g/kg Side-effect Attenuation Prescriptions once a day for two weeks, respectively. Mice in the G-CSF group were given G-CSF (50 μg/kg)by hypodermic injection three days before blood sampling. Levels of WBC, HGB and PLT counts in peripheral bloods of the mice were detected at 7 d and 14 d after the marrow depressed models were established. Results The Side-effect Attenuation Prescriptions slowed down the decline of blood levels of WBC, HGB and PLT induced by CTX ( P<0.05), and accelerated the recovery of WBC and HGB levels compared with model group at 14 d( P<0.05). Conclusion The Side-effect Attenuation Prescriptions can accelerate recovery of WBC, HGB and PLT in peripheral bloods of mice.