Interaction Between GNB3 C825T and ACE I/D Polymorphisms in Pre-eclampsia

Objective To verify the hypothesis if interaction between the G protein β3 subunit (GNB3) C825T polymorphism and angiotensin-I converting enzyme (ACE) insertion/deletion (I/D) could lead to the increased risk of pre-eclampsia. Methods Analyses of ACEand GNB3genotypes were performed in 188 pre-eclamptic patients and 273 normal pregnant controls by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism in Chinese population, respectively. Results The distributions of alleles and genotypes for the GNB3C825T and ACE I/D polymorphisms were not found to be significantly associathed with pre-eclamptic status. No significant interaction of the influence of GNB3T allele and ACEgenotypes on the risk of pre-eclampsia was observed (OR 0.439-1.203, all P >0.05). However, we found that in homozygous 825T genotype carriers with the ACE II genotype in controls diastolic blood pressure (DBP) levels showed highest 〔(77.61±1.26） mmHg (1 mmHg=0.133 kPa)〕 among other three genotype combinations 〔TT/ID, （70.94±1.64） mmHg; CT/ID, （73.15±0.89） mmHg; CT/DD, （72.57±2.14） mmHg〕 (all P GNB3825T allele carriers with theACEI/D polymorphism in pre-eclampsia in Chinese population in Chengdu area. However there is the interaction of the two genes on DBP levels in pregnancy women without pre-eclampsia in the population.