Expression of Fatty Acid Synthase and Adipocyte Fatty Acid-binding Protein and the Relationship with the Clinicopathological Characteristics in Human Infiltrating Ductal Breast Cancer

Objective To study the expression of fatty acid synthase (FASN) and adipocyte fatty acid-binding protein (A-FABP) in human infiltrating ductal breast cancer (IDC) tissues and hunman breast cancer cells and the relationship with the clinicopathogical characteristics. To further explore the relationship between FASN and A-FABP, and the relevance of the invasion in cancer cell. Methods The expression of FASN and A-FABP was detected in 58 cases of human infiltrating ductal breast cancer and 12 cases of human normal breast tissues by immunohistochemistry technique, calculated positive expression percentage according to the number of positive cells percentage and the staining degree of positive sediment. The cell wound-healing assay was applied to detect the invasion of SKBR3 and MCF-7 cells. Western blot was used to detect the expression of FASN and A-FABP in MCF-7 and SKBR3 cells. Results The positive rates of FASN and A-FABP were 8.3% (1/12) and 16.7% (1/6) respectively in 12 cases of normal breast tissues by immunohistochemistry.In 58 cases of IDC tissues, the positive rates of FASN and A-FABP were 72.4% (42／58) and 79.3% (46／58) respectively. The differences of the positive rates of FASN and A-FABP in normal breast and IDC tissues were statistically significant (\P<0.01,\P<0.05). The expression of FASN and A-FABP was up-regulated in the lymph node metastasis positive group and the larger