Effects of LingQiJuanGan Capsule Medicated Serum on Apoptosis of Activated Rat Hepatic Stellate Cells

Objective To investigate the effects of LingQiJuanGan Capsule medicated serum on the apoptosis of and the expression of Bcl-2/Bax in hepatic stellate cell-T6 (HSC-T6) activated by platelet-derived growth factor (PDGF). Methods Preparation of drug-medicated serum. 30 SD rats were randomly divided into three groups. Serum was obtained from the SD rats administrated intragastricly with saline (10 mL/kg, group A), Fufang Biejia Ruangan Tablet solution (1.5 g/kg, group B) and LingQiJuanGan Capsule solution respectively (4.25 g/kg, group C). Detection of cell apoptosis and the expression of Bcl-2/Bax. HSC-T6 cells were activated by 10 ng/mL PDGF and incubated with drug-medicated serum at 200 mL/L. Cell apoptosis and the expression of Bcl-2/Bax were measured by flow cytometry at 0, 12, 24 and 48 h. Results Significantly higher apoptotic rates of HSC-T6 in group B and group C were detected at 12, 24, 48 h compared with that of group A (P<0.05 ), wheraus no significant difference was found between group B and group C (P>0.05). With increase of exposure time, the apoptotic rates of HSC-T6 in group B and group C increased (P<0.05). The levels of Bcl-2 expression in HSC-T6 in group B and C were lower than that of group A (P<0.05) at 12, 24, 48 h (No significant difference between group B and group C, P>0.05). The expression level of Bcl-2 decreased with increased exposure time (P>0.05) in both group B and group C.However, no such trend was observed in group A (P>0.05). In both group B and group C, the expression level of Bax increased in a time-dependent manner. Conclusion LingQiJuanGan Capsule can induce apoptosis of PDGF activated HSC-T6 and inhibit hepatic fibrosis in a time-dependent manner by influencing the expression of Bcl-2/Bax.