The Anti-virus Effect of <i>AY</i>358935 Gene on Vesicular Stomatitis Virus and the Mechanism Study

LI Guang-ping; KE Jian-long; LÜ Pan-pan; ZHU Xi; WANG Cui; WANG Jin-yang; SHEN Xian-ying; XIONG Shao-quan

Volume 50 Issue 4

Jul. 2019

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JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) > 2019 > 50(4): 540-545.

LI Guang-ping, KE Jian-long, LÜ Pan-pan, et al. The Anti-virus Effect of AY358935 Gene on Vesicular Stomatitis Virus and the Mechanism Study[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(4): 540-545.

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The Anti-virus Effect of AY358935 Gene on Vesicular Stomatitis Virus and the Mechanism Study

Department of Oncology, Affiliated Hospital of Chengdu University of TCM, Chengdu 610072, China

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Corresponding author:
XIONG Shao-quan, E-mail:xsquan106@163.com
Received Date: November 03, 2018
Revised Date: March 20, 2019
Available Online: March 16, 2021
Published Date: July 19, 2019

Abstract

Abstract

Objective To explore the anti-virus effect of AY358935 gene cloned by our research team on vesicular stomatitis virus (VSV), and studytheanti-virus mechanism.
Methods HEK293 cells were stably transfected by the AY358935 gene recombinant plasmid pcDNA3.1-AY358935 or pcDNA3.1 blank plasmid respectively. Then VSV was added into the cell wells to infect the above cells at the multiplicity of infection (MOI) of 0.001. The virus titers in the liquid supernatant of the above three groups of cells were detected on different time, and the mortality of cells of each group was tested with trypan blue exclusion test at 24 h post VSV infection. Total RNA was extracted from the cells that stably transfected with target gene for the whole genome-wide cDNA microarray analysis.
Results ① Virus titer:The virus titer in the liquid supernatant of pcDNA-3.1-AY358935 transfection cells group was obviously lower than those in pcDNA-3.1 transfection cell group and blank control cell group at 12 h post infection. The virus titerin the liquid supernatant of three groups were (7.16±2.33)×10⁵ PFU/mL, (6.25±2.05)×10⁶ PFU/mL and (7.75±2.54)×10⁶ PFU/mL respectively at 18 h post infection. At that time, the virus titerin the liquid supernatant of pcDNA3.1-AY358935 group was nearly 10 times lower than those of other two groups (P < 0.01). ②Mortality of cells:The cell mortality of pcDNA3.1-AY358935 group, pcDNA3.1 group and blank group were (35.00±6.68)%, (78.33±15.03)% and (83.34±14.98)% respectively at 24 h post infection.The cell mortality of pcDNA3.1-AY358935 group was significantly decreased comparing with other two groups (P < 0.01). ③Result of genes chip analysis: compared with pcDNA3.1 group, 30 cell genes were up-regulated by more than 3 times in pcDNA3.1-AY358935 group. Among them, the proportion of interferon-activating gene, interferon-effect gene, cytokine and chemokine was 27%, 17%, and 20%, respectively.
Conclusion AY358935 gene hasan anti-VSV effect, and its anti-virus mechanism may involve the interferon-associated natural immune response.
- AY358935 gene,
- VSV,
- Interferon

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[1]	熊绍权, 杨寒朔, 龙奇达.新基因AY358935的生物信息学分析及功能预测.南方医科大学学报, 2010, 2(30):232-238. http://d.old.wanfangdata.com.cn/Periodical/dyjydxxb201002008
[2]	周丹, 刘雪茹, 李涛, 等.小G蛋白Rab5对表达在HEK293细胞上的大电导钙激活钾通道的影响.山东医药, 2017, 57(8):21-24. DOI: 10.3969/j.issn.1002-266X.2017.08.006
[3]	孟越, 谢苗苗, 林振, 等.甲状旁腺素受体真核表达载体的构建体稳定转染HEK293细胞系的建立.南方医科大学学报, 2013, 33(7):956-961. DOI: 10.3969/j.issn.1673-4254.2013.07.04
[4]	蔡鑫泽, 顾卉, 刘彤, 等.P16 hLMO1编码基因重组质粒的构建及蛋白的表达和定位.中国医科大学学报, 2009, 38(11):816-819. http://www.cnki.com.cn/Article/CJFDTotal-ZGYK200911007.htm
[5]	LI XY, KUANG Y, HUANG XJ, et al. Preparation and characterization of a new monoclonal antibody against CXCR4 using lentivirus vector.Int Immunopharmacol, 2016, 36:100-105. DOI: 10.1016/j.intimp.2016.04.020
[6]	薛贻敏, 林风辉, 刘艳丽, 等.白细胞介素17A对柯萨奇B3病毒性心肌炎小鼠病毒复制的影响及其机制.临床心血管病杂志, 2017, 33(7):688-693. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=lcxxgbzz201707019
[7]	程仕彤, 冯鹭, 刘丽娜, 等.干扰素对肝纤维化小鼠肝组织中Foxp3调节性T细胞的影响.中国医科大学学报, 2013, 42(5):402-405. DOI: 10.3969/j.issn.0258-4646.2013.05.005
[8]	GNANADURAI CW, FU ZF. CXCL10 and blood-brain barrier modulation in rabies virus infection. J Oncotarget, 2016, 7 (10):10694-10695. http://cn.bing.com/academic/profile?id=1434a391f39d07cdfd8aa8edaa6ff491&encoded=0&v=paper_preview&mkt=zh-cn
[9]	ZHAO A, LU W, DE LE. Functional synergism of human defensin 5 and human defensin 6. Biochem Biophys Res Commun, 2015, 467(4):967-972. DOI: 10.1016/j.bbrc.2015.10.035
[10]	CURRIE SM, FINDLAY EG, MCFARLANE AJ, et al. Cathelicidins have direct antiviral activity against respiratory syncytial virus in vitro and protective function in vivo in mice and humans. J Immunol, 2016, 196(6):2699-2710. DOI: 10.4049/jimmunol.1502478
[11]	PEEL E, CHENG Y, DJORDJEVIC JT, et al. Cathelicidins in the Tasmanian devil (Sarcophilus harrisii). Sci Rep, 2016, 6:35019. DOI: 10.1038/srep35019
[12]	REBHANDL S, HUEMER M, GREIL R, et al. AID/APOBEC deaminases and cancer. Oncoscience, 2015, 2(4):320-333. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468319/
[13]	LEI Q, GAO B, ZHANG D. Design and preparation of matrine surface-imprinted material and studies on its molecule recognition selectivity. J Biomater Sci Polym Ed, 2016, 27(1):1-21. http://cn.bing.com/academic/profile?id=878b45e3c5342f94a12c286166cad620&encoded=0&v=paper_preview&mkt=zh-cn
[14]	SAFA A, RASHIDINEJAD HR, KHALILI M, et al. Higher circulating levels of chemokines CXCL10, CCL20 and CCL22 in patients with ischemic heart disease. Cytokine, 2016, 83: 147-157[2018-10-10].https://doi.org/10.1016/j.cyto.2015.04.006.
[15]	GRAWENHOFF J, ENGELMAN AN. Retroviral integrase protein and intasome nucleoprotein complex structures. World J Biol Chem, 2017, 8(1):32-44. DOI: 10.4331/wjbc.v8.i1.32
[16]	YIN Y, LIU W, DAI Y. SOCS3 and its role in nassociated diseaseas. Hum Immunol, 2015, 76(10):775-780. DOI: 10.1016/j.humimm.2015.09.037
[17]	CHOW KT, GALE MJ. SnapShot: interferon signaling. Cell, 2015, 163(7): 1808e1[2018-10-10]. https://doi.org/10.1016/j.cell.2015.12.008.
[18]	宪庆, 伍参荣. 2′-5′寡聚腺苷酸合成酶(OAS)的活化及抗病毒作用应用研究进展.中外健康文摘, 2012, 9(15):84-86. DOI: 10.3969/j.issn.1672-5085.2012.15.056
[19]	HUANG Y, HUANG X, CAI J, et al. Identification of orange-spotted grouper (Epinephelus coioides) interferon regulatory factor 3 involved in antiviral immune response against fish RNA virus. Fish Shellfish Immunol, 2015, 42 (2):345-352. DOI: 10.1016/j.fsi.2014.11.025
[20]	HENKES LE, PRU JK, ASHLEY RL, et al. Embryo mortality in Isg15^-/-mice is exacerbated by environmental stress. Biol Reprod, 2015, 92(2): 36[2018-10-10]. https://doi.org/10.1095/biolreprod.114.122002.
[21]	DONNELLY RP, KOTENKO SV. Interferon-lambda:a new addition to an old family. J Interferon Cytokine Res, 2010, 30(8):555-564. DOI: 10.1089/jir.2010.0078
[22]	GIBBERT K, SCHLAAKJ F, YANG D. IFN-alpha subtypes:distinct biological activities in anti-viral therapy. Br J Pharmacol, 2013, 168(5):1048-1058. DOI: 10.1111/bph.12010
[23]	DONNELLY RP, KOTENKO SV. Interferon-lambda:a new addition to an old family. J Interferon Cytokine Res, 2010, 30(8):555-564. DOI: 10.1089/jir.2010.0078
[24]	DE VEER MJ, HOLKO M, FREVEL M, et al. Functional classification of interferon-stimulated genes identified using microarrays. J Leukoc Biol, 2001, 69(6):912-920. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=2c14ffc803cd56d3ef10b7ff278c5f96
[25]	SCHNEIDER WM, CHEVILLOTTE MD, RICE CM. Interferon- stimulated genes: a complex web of host defenses. Annu Rev Immunol, 2014, 32: 513-545[2018-10-10].https://doi.org/10.1146/annurev-immunol-032713-120231.
[26]	CHEN Z, MARK C, TIEN YH, et al. Interferon-induced ISG15 pathway:an ongoing virus-host battle. Trends Microbiol, 2013, 21(4):181-186. DOI: 10.1016/j.tim.2013.01.005

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The Anti-virus Effect of AY358935 Gene on Vesicular Stomatitis Virus and the Mechanism Study

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