In vitro and in vivo Antitumor Effects of Fisetin and Fisetin Nanopartical on Ovarian Cancer

Objective To explore the effects of fisetin and fisetin nanopartical on anti-proliferation and apoptosis induction of human ovarian cancer cell line SKOV3. Methods The MTT assay was used to determine the survival rate of SKOV3 cell through apoptosis induction after fisetin and fisetin nanopartical treatment, and the drug concentration of the next experiment was selected. 15 nude mice vaccinated SKOV3 cells mixture mode randomly into three groups, treatment began on the fifth day, every 3 d injection times, each time 0.2 mL, a total of 12 times. Group A for the control (50 mL/L glucose solution), group B for fisetin (1.25 mg/kg), group C for fisetin nanopartical (1.25 mg/kg). After the treatment, tumor quality was recorded, and histopathological lesions were observed by HE staining on heart, liver, spleen, lung and kidney sections. The proliferation of SKOV3 cells was detected by Ki67 immunohistochemical staining in tumor tissue sections, and apoptosis of SKOV3 cells was detected by TUNEL assay. Results MTT test indicated that fisetin and fisetin nanopartical had inhibition effect on ovarian cancer cell in a dose-dependent manner. The half-maximal inhibitory concentration (IC₅₀) value for fisetin and fisetin nanopartical were 125-250 μg/mL and 62.5-125 μg/mL. Therefore, the working concentration was selected as 125 μg/mL. 11 mice were completed and survived until the end of the experiment (n=3 in group A, n=4 in group B, n=4 in group C). Group A nude mice abdominal cavity in cauliflower shape tumor nodules, B and C group only tiny granule tumor nodules, B and C group of intraperitoneal tumor mass is quite (P>0.05), but were lower than group A (P<0.05). No obvious damage was found in the hepatocytes, liver, spleen, lung, and kidney slices. Compared with group A, Ki67 index in group B and group C decreased, and cell apoptosis rate increased (P<0.05), but there was no significant difference between group B and group C (P>0.05).After treatment of fisetin and fisetin nanopartical, heart, liver, spleen, lung and kidney are safe without damage. Conclusion Fisetin and fisetin nanopartical have the effect of anti-ovarian cancer cells, and no organ damage has been found in both.