Effect of Chlorogenic Acid on Hepatic Stellate Cell Proliferation, Generation and Degradation of Extracelluar Matrix

Objective To investigate the effects of chlorogenic acid (CGA) on hepatic stellate cell proliferation and the expression and secretion of Collagen Ⅰ, Collagen Ⅲ, tissue inhibitors of metalloproteinase-1 (TIMP-1) and matrix metalloproteinase-2 (MMP-2). Methods An immortalized rat hepatic stellate cell (HSC) line was cultured In vitro. The cells were divided into 5 groups: control group; platelet-derived growth factor (PDGF) (10 ng/mL), PDGF+CGA (12.5 μg/mL), PDGF+CGA (25 μg/mL), PDGF+CGA (50 μg/mL) and CGA (50 μg/mL) group. After 24 hours treatment, the proliferation of HSC was detected by MTT method. The mRNA expression of Collagen Ⅰ, Collagen Ⅲ, TIMP-1 and MMP-2 were detected by RT-PCR. The protein levels of Collagen Ⅰ, Collagen Ⅲ, TIMP-1 and MMP-2 in the culture supernatant of HSC were measured by ELISA. Results PDGF increased the hepatic stellate cell proliferation, the mRNA expression and the protein levels of Collagen Ⅰ, Collagen Ⅲ and TIMP-1 (P<0.05), which were significantly decreased by CGA (P<0.05). However, CGA had no significant influence on the expression of MMP-2. Conclusion The antifibrotic effect of CGA may be related with the inhibition of hepatic stellate cell proliferation and generation of extracelluar matrix and promotion of extracelluar matrix degradation.