Quality Control and Biological Assessment of Automated Synthesized 3′-deoxy-3′- 18F-fluorothymidine using CFN-MPS-200 Module

【Abstract】 Objective To synthesize 3′-deoxy-3′-18F-fluorotyhymidine(18F-FLT) using CFN-MPS-200 automatic synthesis module, and evaluate its distribution in Wistar rats. Methods We used 3-N-Boc-5′-O-dimethoxytrityl-3′-O-nosyl-thymidine (Boc-FLT)-percursor as raw material to synthesize 18F-FLT without residual solvents. Its radiochemical purity was confirmed with radio-HPLC and thin layer chromatography (TLC). Normal Wistar rats were injected with 18F-FLT and underwent PET scanning. Results The entire preparation procedure took about 60 min, which resulted in a radio chemical yield of (24±5)% (after attenuation correction, n =20) and radiochemical purity of over 99%, with 1.11×109 Bq/mL specific activity. The 18F-FLT solution was colorless and had a pH value between 7.0-8.0. 18F-FLT was mainly concentrated in the kidney, bladder, liver, bone marrow and Liver of normal Wistar rats. Conclusion Automated synthesis of 18F-FLT using CFN-MPS-200 is a stable method, with high yield, safety without solvent, and acceptable quality.