Growth Regulation of Factor Inhibiting Hypoxia-Inducible Factor in Renal Carcinoma Cells

Objective To detect the expression and growth regulation effects of the factor inhibiting hypoxia-inducible factor (FIH-1) in renal carcinoma cells. Methods The expressions of FIH-1, hypoxia inducible factor (HIF) and vascular endothelial growth factor (VEGF) were examined in OS-RC-2 and 786-0 cell lines via real-time PCR (RT-PCR) and Western blot. Overexpression plasmid of FIH-1 were designed and constructed as EX-V1104-M45-FIH-1. After the transfection of the plasmid into OS-RC-2 and 786-0 cells, the expression levels of FIH-1, HIF and VEGF were measured by RT-PCR and Western blot, while cell proliferation was tested by MTT. Results The expressions of FIH-1, HIF-1α, HIF-2α and VEGF were detected in OS-RC-2 cells, and FIH-1, HIF-2α and VEGF were detected in 786-0 cells. After the transfection of EX-V1104-M45-FIH-1,the level of FIH-1 was increased in OS-RC-2, while the levels of HIF-2α, HIF-1α and VEGF were decreased. MTT showed that FIH-1 plasmid transfection decreased the cell proliferation of OS-RC-2, but did not show inhibition on 786-0 cells proliferation. Conclusion FIH-1/HIF-1α/VEGF signaling pathway exists in renal carcinoma cell lines.