The Comparison Between Extraction Methods of Exosomes from Ascites of Colorectal Cancer

Objective To compare the two different methods to isolate the exosome from the ascites of colorectal cancer (CRC) patient and find the efficient one. Methods Exosome from the ascites of CRC patient were isolated by two different methods: density gradient exosome isolation (DG-Exo) and Exo-Quick isolation, and followed by identification with transmission electron microscopy observation and Western blot analysis. And then, Nanodrop was used for protein quantification. Results Exosome were isolated by both of the two methods. The protein concentration of the exosome isolated by the Exo-Quick isolation were higher than that of DG-Exo. Conclusion Exo-Quick isolation can obtain higher purity and more complete exosome from the ascites.