Effects of IL-1β Pretreated Adipose Tissue-derived Mesenchymal Stem Cells on VEGF Secretion and Macrophage M₂ Polarization

ObjectiveTo investigate the effects of interleukin (IL)-1β preteated adipose tissue-derived mesenchymal stem cells (ADMSCs) on vascular endothelial growth factor (VEGF) secretion and macrophages M₂ polarization. MethodsAfter IL-1β pretreated ADMSC for 24 h,the expression of cyclooxygenase-2 (COX-2) was detected using Western blot,and the secretions of prostaglandin E₂ (PGE₂) and VEGF were measured by ELISA method. Conditioned media collected from ADMSCs was used for culturing PMA-U937 macrophages for 72 h,and the expressions of CD163 and IL-10 mRNAs were detected using qRT-PCR. After inhibition of COX-2 expression in ADMSCs by Lentivirus silencing,secretion of VEGF and CD163 and IL-10 mRNA expressions were detected. ResultsIL-1β pretreating ADMSCs increased the expression level of COX-2,enhanced PGE₂ and VEGF secretions (P<0.05). In addition,the mRNA levels of CD163 and IL-10 were upregulated when ADMSCs were pretreated by IL-1β in PMA-U937 cells. After downregulation of COX-2 in ADMSCs,the secretion of VEGF was suppressed,and the mRNA levels of CD163 and IL-10 were also significantly down-regulated. ConclusionIL-1β pretreatment enhanced the secretion of PGE₂ and VEGF,and induced macrophage M₂ polarization,which depended on COX-2-PGE₂ signal pathway.