The Protective Effect of Olanzapine on the Hippocampal Neuron of Depression Model Rats via Inhibiting NLRP3 Inflammasome Activation

YUE Ling-feng; ZHONG Zhao-xi; MA Jing; WANG Ning

Volume 50 Issue 5

Sep. 2019

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JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) > 2019 > 50(5): 672-678.

YUE Ling-feng, ZHONG Zhao-xi, MA Jing, et al. The Protective Effect of Olanzapine on the Hippocampal Neuron of Depression Model Rats via Inhibiting NLRP3 Inflammasome Activation[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(5): 672-678.

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The Protective Effect of Olanzapine on the Hippocampal Neuron of Depression Model Rats via Inhibiting NLRP3 Inflammasome Activation

1.
The Forth Department, the Second Affiliated Hospital of Xinxiang Medical University, Xinxiang 453002, China
2.
Department of Mood Disorder, the Second Affiliated Hospital of Xinxiang Medical University, Xinxiang 453002, China
3.
The Third Department, the Second Affiliated Hospital of Xinxiang Medical University, Xinxiang 453002, China

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Corresponding author:
ZHONG Zhao-xi . E-mail:zzxzzx9268@tom.com
Received Date: February 18, 2019
Revised Date: May 28, 2019
Available Online: March 16, 2021
Published Date: September 19, 2019

Abstract

Abstract

Objective To determine the impact olanzapine (OLA) on the hippocampal neuron of model rats with depression.
Methods Rats were divided into five groups: control, chronic unpredicted stress (CUS), OAL (0.5, 1, 2 mg/kg), si-Atg5, and OAL (2 mg/kg)+si-Atg5. Open field and sucrose preference tests were performed to evaluate rat behaviors. Cell apoptosis was detected with Tunnel. The concentrations of interleukin (IL)-1β and IL-18 were determined by ELISA. The expressions of cleaved Caspase-3, cleaved Caspase-9, LC3, Beclin1, P62, NLRP3 and cleaved Caspase-1 were measured by Western blot.
Results OAL (0.5, 1, 2 mg/kg) increased the total moving distance, sucrose consumption and preference rate of CUS rats, and decreased serum IL-18, cell apoptosis and the expressions of cleaved Caspase-9, cleaved Caspase-1 and NLRP3 in the CA3 region of hippocampus. Although OAL (1, 2 mg/kg) decreased the expression of cleaved Caspase-3 and serum IL-1β, OAL (0.5 mg/kg) showed no detectable effects. Si-Atg5 decreased the total moving distance, sucrose consumption and preference rate of CUS rats, enhanced the expressions of cleaved Caspase-3, cleaved Caspase-9, cleaved Caspase-1 and NLRP3, and weakened the effect of OAL (2 mg/kg). OAL (0.5, 1, 2 mg/kg) also increased the LC3Ⅱ/LC3Ⅰ ratio and the expression of Beclin1 in the CA3 region of hippocampus. OAL (1, 2 mg/kg) reduced the expression of p62, but not when it was reduced to 0.5 mg/kg. Si-Atg5 reduced the LC3Ⅱ/LC3Ⅰ ratio and the expression of Beclin1, and weakened the function of OAL (2 mg/kg).
Conclusion OAL can protect the hippocampal neuron of CUS rats via inhibiting NLRP3 inflammasome activation.
- Olanzapine,
- Depression,
- NLRP3 inflammasome

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[1]	SONG W, LI H, GUO T, et al. Effect of affective reward on cognitive event-related potentials and its relationship with psychological pain and suicide risk among patients with major depressive disorder. Suicide Life Threat Behav, 2018 Nov 2[2018-12-19]. https://doi.org/10.1111/sltb.12524.
[2]	SOLEM S, HAGEN R, WANG C E, et al. Metacognitions and mindful attention awareness in depression:a comparison of currently depressed, previously depressed and never depressed individuals. Clin Psychol Psychother, 2017, 24(1):94-102. DOI: 10.1002/cpp.1983
[3]	DAVIES S J C, MULSANT B H, FLINT A J, et al. SSRI-antipsychotic combination in psychotic depression:Sertraline pharmacokinetics in the presence of olanzapine, a brief report from the STOP-PD study. Hum Psychopharmacol, 2016, 31(3):252-255. https://www.ncbi.nlm.nih.gov/pubmed/27060853
[4]	CITROME L. Treatment of bipolar depression:making sensible decisions. CNS Spectr, 2014, 19 Suppl 1:4-11. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_PM25407667
[5]	TOHEN M, VIETA E, CALABRESE J, et al. Efficacy of olanzapine and olanzapine-fluoxetine combination in the treatment of bipolarⅠ depression. Arch Gen Psychiatry, 2003, 60(11):1079-1088. DOI: 10.1001/archpsyc.60.11.1079
[6]	NINAN I, KULKARNI S K. Preferential inhibition of dizocilpine-induced hyperlocomotion by olanzapine. Eur J Pharmacol, 1999, 368(1):1-7. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_PM10096763
[7]	BANERJEE R, HAZRA S, GHOSH A K, et al. Chronic administration of bacopa monniera increases BDNF protein and mRNA expressions:a study in chronic unpredictable stress induced animal model of depression. Psychiatry Investig, 2014, 11(3):297-306. DOI: 10.4306/pi.2014.11.3.297
[8]	WILLIAMS L J, PASCO J A, JACKSON H, et al. Depression as a risk factor for fracture in women: a 10 year longitudinal study. J Affect Disord, 2016, 192:34-40. DOI: 10.1016/j.jad.2015.11.048
[9]	CHHIBBER A, WOODY S K, KARIM RUMI M A, et al. Estrogen receptor beta deficiency impairs BDNF-5-HT2A signaling in the hippocampus of female brain: a possible mechanism for menopausal depression. Psychoneuroendo-crinology, 2017, 82:107-116. DOI: 10.1016/j.psyneuen.2017.05.016
[10]	ASAOKA N, NAGAYASU K, NISHITANI N, et al. Olanzapine augments the effect of selective serotonin reuptake inhibitors by suppressing GABAergic inhibition via antagonism of 5-HT(6) receptors in the dorsal raphe nucleus. Neuropharmacology, 2015, 95:261-268. DOI: 10.1016/j.neuropharm.2015.03.032
[11]	QIN T, FANG F, SONG M, et al. Umbelliferone reverses depression-like behavior in chronic unpredictable mild stress-induced rats by attenuating neuronal apoptosis via regulating ROCK/Akt pathway. Behav Brain Res, 2017, 317:147-156. DOI: 10.1016/j.bbr.2016.09.039
[12]	LIU W X, WANG J, XIE Z M, et al. Regulation of glutamate transporter 1 via BDNF-TrkB signaling plays a role in the anti-apoptotic and antidepressant effects of ketamine in chronic unpredictable stress model of depression. Psychopharmacology, 2016, 233(3):405-415. DOI: 10.1007/s00213-015-4128-2
[13]	KIM N R, PARK S W, LEE J G, et al. Protective effects of olanzapine and haloperidol on serum withdrawal-induced apoptosis in SH-SY5Y cells. Prog Neuropsychopharmacol Biol Psychiatry, 2008, 32(3):633-642. DOI: 10.1016/j.pnpbp.2007.11.002
[14]	WANG H, XU H, DYCK L E, et al. Olanzapine and quetiapine protect PC12 cells from beta-amyloid peptide(25-35)-induced oxidative stress and the ensuing apoptosis. J Neurosci Res, 2005, 81(4):572-580. DOI: 10.1002/jnr.20570
[15]	BELMOKHTAR C A, HILLION J, SEGALBENDI-RDJIAN E. Staurosporine induces apoptosis through both caspase-dependent and caspase-independent mechanisms. Oncogene, 2001, 20(26):3354-3362. DOI: 10.1038/sj.onc.1204436
[16]	KIECOLT-GLASER J K, DERRY H M, FAGUNDES C P. Inflammation:depression fans the flames and feasts on the heat. Am J Psychiatry, 2015, 172(11):1075-1091. DOI: 10.1176/appi.ajp.2015.15020152
[17]	VALERA E, UBHI K, MANTE M, et al. Antidepressants reduce neuroinflammatory responses and astroglial alpha-synuclein accumulation in a transgenic mouse model of multiple system atrophy. Glia, 2014, 62(2):317-337. DOI: 10.1002/glia.22610
[18]	THOMAS J, KHANAM R, VOHORA D. Activation of indoleamine 2, 3-dioxygenase pathway by olanzapine augments antidepressant effects of venlafaxine in mice. Psychiatry Res, 2017, 258:444-448. DOI: 10.1016/j.psychres.2017.08.083
[19]	ZHONG Z, SANCHEZ-LOPEZ E, KARIN M. Autophagy, NLRP3 inflammasome and auto-inflammatory/immune diseases. Clin Exp Rheumatol, 2016, 34 (4 Suppl 98):12-16. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=3fd5a2cf7068d1e8f956900b74ac6c87
[20]	DU R H, TAN J, SUN X Y, et al. Fluoxetine inhibits NLRP3 inflammasome activation: implication in depression. Int J Neuropsychopharmacol, 2016, 19(9).pii: pyw037[2018-10-13].doi: 10.1093/ijnp/pyw037.print2016sep.
[21]	ALCOCER-GOMEZ E, CASAS-BARQUERO N, WILLIAMS M R, et al. Antidepressants induce autophagy dependent-NLRP3-inflammasome inhibition in major depressive disorder. Pharmacol Res, 2017, 121:114-121. DOI: 10.1016/j.phrs.2017.04.028
[22]	HEISEKE A, AGUIB Y, RIEMER C, et al. Lithium induces clearance of protease resistant prion protein in prion-infected cells by induction of autophagy. J Neurochem, 2009, 109(1):25-34. DOI: 10.1111/j.1471-4159.2009.05906.x
[23]	KWON D H, KIM L. pH-dependent regulation of SQSTM1/p62 during autophagy. Autophagy, 2019, 15(1):180-181. DOI: 10.1080/15548627.2018.1532264
[24]	SHEN L, YANG Y, OU T, et al. Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy. J Lipid Res, 2017, 58(9):1808-1821. DOI: 10.1194/jlr.M075879
[25]	DAI J, ZHANG X, LI L, et al. Autophagy inhibition contributes to ROS-producing NLRP3-dependent inflammasome activation and cytokine secretion in high glucose-induced macrophages. Cell Physiol Biochem, 2017, 43(1):247-256. DOI: 10.1159/000480367
[26]	CHIU Y H, HSU S H, HSU H W, et al. Human non-small cell lung cancer cells can be sensitized to camptothecin by modulating autophagy. Int J Oncol, 2018, 53(5):1967-1979.

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The Protective Effect of Olanzapine on the Hippocampal Neuron of Depression Model Rats via Inhibiting NLRP3 Inflammasome Activation

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