Welcome to JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) July 14, 2025
| Citation: |
RONG Xiao, XIANG Xi, ZHAO Yicheng, et al. Experimental Study on Biomimetic Curcumin-Mediated Sonodynamic Therapy of Melanoma[J]. Journal of Sichuan University (Medical Sciences), 2024, 55(5): 1159-1165. DOI: 10.12182/20240960108
|
To study the role of curcumin-mediated sonodynamic therapy in the treatment of malignant melanoma, and to provide a new strategy for the treatment of malignant melanoma.
The ultrasonic sound and vibration method was applied to coat curcumin with mouse melanoma cell membrane, thereby forming biomimetic curcumin. The morphology of biomimetic curcumin was observed by transmission electron microscope. Flow cytometry was used to analyze the effect of biomimetic curcumin in terms of in vitro targeting, apoptosis, and intracellular reactive oxygen species (ROS) production. The in vivo experiment was divided into control group, US group, turmeric group, imitation turmeric group, and imitation turmeric+US group, with 3 mice in each group. The in vivo safety of biomimetic curcumin was evaluated by HE staining. In addition, HE, CD31, Ki67, and TUNEL stainings were performed to evaluate the in vivo anti-melanoma therapeutic effect of ultrasound combined with biomimetic curcumin.
The biomimetic curcumin had a generally uniform morphology and possessed a core-shell structure. Flow cytometry analysis performed with FlowJo showed that the biomimetic curcumin could be effectively taken up by melanoma cells. The apoptosis rate was (10.30±0.61)% in the control group, (10.41±3.13)% in the ultrasound group, (24.97±1.38)% in the curcumin group, (31.39±3.84)% in the biomimetic curcumin group, and (40.89±0.79)% in the biomimetic curcumin and ultrasound combination group. The apoptosis rate in the biomimetic curcumin and ultrasound combination group was higher than those in the other groups (P<0.05). The results of ROS flow cytometry showed that, compared with the control group, the ultrasound group demonstrated almost no increase in the fluorescence intensity, while the other groups showed an increase in the fluorescence intensity to varying degrees. There was no significant difference in the fluorescence intensity between the biomimetic curcumin group ([1.10±0.38]%) and the curcumin group ([0.73±0.26]%) (P>0.05). The fluorescence intensity of the biomimetic curcumin and ultrasound combination group ([3.35±0.04]%) was higher than those of the other groups (P<0.05). HE staining showed no obvious abnormalities in the morphology of heart, liver, spleen, lung, and kidney tissues in any of the treatment groups. HE staining showed the most significant changes in cell morphology in the biomimetic curcumin and ultrasound combination group, followed by the biomimetic curcumin group and the curcumin group. No obvious abnormalities in tumor cell morphology were observed in the ultrasound group. According to the respective results of CD31 staining, Ki67 staining, and TUNEL staining, the biomimetic curcumin and ultrasound combination group had the largest brown area, the highest number of red fluorescence, and the highest number of green fluorescence, followed by the biomimetic curcumin group and the curcumin group.
The biomimetic curcumin displays uniform morphology, a core-shell structure, and good targeting properties. When it is used in combination with ultrasound, biomimetic curcumin demonstrates a good anti-tumor therapeutic effect both in vivo and in vitro.
| [1] |
VISNJIC A, KOVACEVIC P, VELICKOV A, et al. Head and neck cutaneous melanoma: 5-year survival analysis in a Serbian university center. World J Surg Oncol, 2020, 18(1): 312. doi: 10.1186/s12957-020-02091-4.
|
| [2] |
LIM Y, LEE J, LEE D Y. Is the survival rate for acral melanoma actually worse than other cutaneous melanomas? J Dermatol, 2020, 47(3): 251–256. doi: 10.1111/1346-8138.15201.
|
| [3] |
CONIC R Z, CABRERA C I, KHORANA A A, et al. Determination of the impact of melanoma surgical timing on survival using the National Cancer Database. J Am Acad Dermatol, 2018, 78(1): 40–46.e7. doi: 10.1016/j.jaad.2017.08.039.
|
| [4] |
COIT D G, THOMPSON J A, ALBERTINI M R, et al. Cutaneous Melanoma, Version 2.2019, NCCN Clinical Practice Guidelines in Oncology. J Natl Compr Canc Netw, 2019, 17(4): 367–402. doi: 10.6004/jnccn.2019.0018.
|
| [5] |
CHAMPIAT S, DERCLE L, AMMARI S, et al. Hyperprogressive disease is a new pattern of progression in cancer patients treated by anti-PD-1/PD-L1. Clin Cancer Res, 2017, 23(8): 1920–1928. doi: 10.1158/1078-0432.CCR-16-1741.
|
| [6] |
XU M M, ZHOU L Q, ZHENG L, et al. Sonodynamic therapy-derived multimodal synergistic cancer therapy. Cancer Lett, 2021, 497: 229–242. doi: 10.1016/j.canlet.2020.10.037.
|
| [7] |
OUYANG J, TANG Z M, FAROKHZAD N, et al. Ultrasound mediated therapy: recent progress and challenges in nanoscience. Nano Today, 2020, 35: 100949. doi: 10.1016/j.nantod.2020.100949.
|
| [8] |
HE M Y, WANG M Y, XU T, et al. Reactive oxygen species-powered cancer immunotherapy: current status and challenges. J Control Release, 2023, 356: 623–648. doi: 10.1016/j.jconrel.2023.02.040.
|
| [9] |
SON S, KIM J H, WANG X W, et al. Multifunctional sonosensitizers in sonodynamic cancer therapy. Chem Soc Rev, 2020, 49(11): 3244–3261. doi: 10.1039/c9cs00648f.
|
| [10] |
XING X J, ZHAO S J, XU T, et al. Advances and perspectives in organic sonosensitizers for sonodynamic therapy. Coord Chem Rev, 2021, 445: 214087. doi: 10.1016/j.ccr.2021.214087.
|
| [11] |
LIN X H, SONG J B, CHEN X Y, et al. Ultrasound-activated sensitizers and applications. Angew Chem Int Ed Engl, 2020, 59(34): 14212–14233. doi: 10.1002/anie.201906823.
|
| [12] |
ALAGAWANY M, FARAG M R, ABDELNOUR S A, et al. Curcumin and its different forms: a review on fish nutrition. Aquaculture, 2021, 532: 736030. doi: 10.1016/j.aquaculture.2020.736030.
|
| [13] |
SHANMUGAM M K, RANE G, KANCHI M M, et al. The multifaceted role of curcumin in cancer prevention and treatment. Molecules, 2015, 20(2): 2728–2769. doi: 10.3390/molecules20022728.
|
| [14] |
PATRA S, PRADHAN B, NAYAK R, et al. Chemotherapeutic efficacy of curcumin and resveratrol against cancer: chemoprevention, chemoprotection, drug synergism and clinical pharmacokinetics. Semin Cancer Biol, 2021, 73: 310–320. doi: 10.1016/j.semcancer.2020.10.010.
|
| [15] |
SOWA-KASPRZAK K, JÓZKOWIAK M, OLENDER D, et al. Curcumin-Triterpene Type Hybrid as Effective Sonosensitizers for Sonodynamic Therapy in Oral Squamous Cell Carcinoma. Pharmaceutics, 2023, 5(7): 2008. doi: 10.3390/pharmaceutics15072008.
|
| [16] |
TABANELLI R, BROGI S, CALDERONE V. Improving curcumin bioavailability: current strategies and future perspectives. Pharmaceutics, 2021, 13(10): 1715. doi: 10.3390/pharmaceutics13101715.
|
| [17] |
YANG D M, TANG Y J, ZHU B H, et al. Engineering cell membrane-cloaked catalysts as multifaceted artificial peroxisomes for biomedical applications. Adv Sci (Weinh), 2023, 10(17): e2206181. doi: 10.1002/advs.202206181.
|
| [18] |
XU K, ZOU Y N, LIN C C, et al. Cascade catalysis nanozyme for interfacial functionalization in combating implant infections associated with diabetes via sonodynamic therapy and adaptive immune activation. Biomaterials, 2024, 311: 122649. doi: 10.1016/j.biomaterials.2024.122649.
|
| [19] |
HAN Y, ZHANG H, ZHAO H T, et al. Nanoparticle encapsulation using self-assembly abietic acid to improve oral bioavailability of curcumin. Food Chem, 2024, 436: 137676. doi: 10.1016/j.foodchem.2023.137676.
|
| [20] |
OMIDIAN H, WILSON R L, CHOWDHURY S D. Enhancing therapeutic efficacy of curcumin: advances in delivery systems and clinical applications. Gels, 2023, 9(8): 596. doi: 10.3390/gels9080596.
|
| [21] |
XIONG J, WU M, CHEN J, et al. Cancer-erythrocyte hybrid membrane-camouflaged magnetic nanoparticles with enhanced photothermal-immunotherapy for ovarian cancer. ACS Nano, 2021, 15(12): 19756–19770. doi: 10.1021/acsnano.1c07180.
|
| [22] |
RAO L, BU L L, CAI B, et al. Cancer cell membrane-coated upconversion nanoprobes for highly specific tumor imaging. Adv Mater, 2016, 28(18): 3460–3466. doi: 10.1002/adma.201506086.
|
| [23] |
YUAN H M, ZHANG L Y, MA T, et al. Spiky cascade biocatalysts as peroxisome-mimics for ultrasound-augmented tumor ablation. ACS Appl Mater Interfaces, 2022, 14(14): 15970–15981. doi: 10.1021/acsami.1c25072.
|
| [24] |
AN X Q, YU W F, LIU J B, et al. Oxidative cell death in cancer: mechanisms and therapeutic opportunities. Cell Death Dis, 2024, 15(8): 556. doi: 10.1038/s41419-024-06939-5.
|
| [25] |
TORRES-CABALA C, LI-NING-TAPIA E, HWU W J. Pathology-based biomarkers useful for clinical decisions in melanoma. Arch Med Res, 2020, 51(8): 827–838. doi: 10.1016/j.arcmed.2020.09.008.
|
| [26] |
SIMON L, ZINI A, DYACHENKO A, et al. A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome. Asian J Androl, 2017, 19(1): 80–90. doi: 10.4103/1008-682X.182822.
|
OPEN ACCESS This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (CC BY-NC 4.0). In other words, the full-text content of the journal is made freely available for third-party users to copy and redistribute in any medium or format, and to remix, transform, and build upon the content of the journal. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may not use the content of the journal for commercial purposes. For more information about the license, visit https://creativecommons.org/licenses/by-nc/4.0
Official website of Journal of Sichuan University (Medical Sciences)
WeChat official account of Journal of Sichuan University(Medical Sciences)
WeChat subscription account of Journal of Sichuan University(Medical Sciences)
Home
DownLoad: