A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning

HU Hui; DU Yu

doi:10.12182/20231160601

Volume 54 Issue 6

Dec. 2023

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JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) > 2023 > 54(6): 1219-1226. > DOI: 10.12182/20231160601

HU Hui, DU Yu. A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning[J]. Journal of Sichuan University (Medical Sciences), 2023, 54(6): 1219-1226. DOI: 10.12182/20231160601

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A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning

HU Hui^{1, 2, 3},
DU Yu^{1, 2, 3, ,}

1.
Department of Emergency and Critical Care Medicine,West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China
2.
Department of Occupational and Environmental Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China
3.
Health Emergency Management Research Center, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu 610041, China

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Corresponding author:
DU Yu, E-mail: 456duyu@163.com
Received Date: December 12, 2022
Revised Date: November 12, 2023
Available Online: December 19, 2023
Published Date: December 24, 2023

Abstract

Abstract

Objective To analyze the plasma metabolomic features of patients suffering from acute diquat (DQ) poisoning and to explore the molecular mechanism and potential biomarkers of DQ poisoning.
Methods A total of 7 patients suffering from acute DQ poisoning were enrolled in the DQ poisoning group. The poisoning of these patients occurred within a 12-h window at the time of enrollment. Meanwhile, 7 healthy immediate family members of the patients were enrolled as the normal controls. Liquid chromatography-mass spectrometry (LC-MS) was used to perform non-targeted metabolomic profiling of the plasma samples and to screen and identify differential metabolites and metabolic pathways.
Results A total of 104 metabolites were screened and identified (P<0.05 and the variable importance in the projection [VIP]>1). Compared with those of the control group, 61 metabolites, such as sorbitol and galactitol, were up-regulated, and 43 metabolites, such as myo-inositol and gamma-glutamylcysteine, were down-regulated in the DQ poisoning group. Pathway enrichment analysis revealed changes in 11 metabolic pathways, including those for galactose metabolism and linoleic acid metabolism (P<0.05).
Conclusion Metabolomics analysis of plasma samples from DQ poisoning patients shows that DQ mainly interferes with the metabolism of energy, amino acids, and lipids, thus causing metabolic disorders. Some potential biomarkers closely associated with oxidative stress and organ damage of the liver, kidney, and nervous system have been identified.
- Diquat,
- Poisoning,
- Metabolomics,
- Plasma

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