Preliminary Study on Microvasculature Normalization Induced by Peritumoral Electroacupuncture in Mice With Breast Cancer Xenografts

CHANG Xin; LU Tao; HUANG Jinchang

doi:10.12182/20230960401

Volume 54 Issue 5

Oct. 2023

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JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) > 2023 > 54(5): 972-977. > DOI: 10.12182/20230960401

CHANG Xin, LU Tao, HUANG Jinchang. Preliminary Study on Microvasculature Normalization Induced by Peritumoral Electroacupuncture in Mice With Breast Cancer Xenografts[J]. Journal of Sichuan University (Medical Sciences), 2023, 54(5): 972-977. DOI: 10.12182/20230960401

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Preliminary Study on Microvasculature Normalization Induced by Peritumoral Electroacupuncture in Mice With Breast Cancer Xenografts

CHANG Xin^1,,
LU Tao²,
HUANG Jinchang^{3, 4, ,}

1.
The Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing 100029, China
2.
College of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China
3.
Department of Acupuncture and Minimally Invasive Oncology, The Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing 100029, China
4.
Institute of Acupuncture and Moxibustion in Cancer Care, Beijing University of Chinese Medicine, Beijing 100029, China

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Corresponding author:
HUANG Jinchang, E-mail: zryhhuang@163.com
Received Date: May 08, 2023
Revised Date: September 04, 2023
Available Online: October 12, 2023
Published Date: October 12, 2023

Abstract

Abstract

Objective To observe the effect of peritumoral electroacupuncture on the induction of vascular normalization in a mouse breast cancer model.
Methods A subcutaneous graft model of breast cancer was established with 4T1 breast cancer cell line in female BALB/c mice aged 6-8 weeks. The mice were randomly assigned to three groups, a tumor-bearing group (TG), peritumoral electroacupuncture tumor-bearing group (EATG), and bevacizumab tumor-bearing group (BTG), with 18 mice in each group. The TG mice did not receive any intervention, the EATG mice received peritumoral electroacupuncture for 30 minutes, and the BTG mice were intraperitoneally injected with bevacizumab at 10mg/kg. Immunofluorescence was performed to assess the expression of CD31/alpha smooth muscle actin (α-SMA) and hypoxia-inducible factor 1-alpha (HIF-1α) in the tumor tissue at various points of time, including before intervention and 3 days and 5 days after intervention. Then, 3 days after intervention, observation of morphological changes of the microvessels in the tumor tissue was performed through Hematoxylin and Eosin (HE) staining and scanning electron microscope.
Results There was no significant difference in the expression of CD31, α-SMA, and HIF-1α in the tumor tissues of all groups before experimental intervention (P>0.05). On day 3 of the experimental interventions, the CD31 and HIF-1α expression levels in the tumor tissues of the EATG and BTG mice were significantly reduced (P<0.01), while α-SMA expression levels were significantly increased (P<0.01) in both groups. On day 5 of the experimental interventions, the CD31 and HIF-1α expression levels in the tumor tissues of the EATG and BTG mice were still significantly lower than those in the TG mice (P<0.01), while the α-SMA expression level was significantly higher than that in the TG group (P<0.05). On day 3 of the experimental interventions, H&E staining showed visible microvessels in the tumor tissues of all 3 groups. In addition, scanning electron microscopic observation showed that the tumor microvessel walls of the TG mice were rough and defective, and that obvious deformities appeared in the lumen. In contrast, the walls of the microvessels of the EATG and BTG mice were generally intact and there was no obvious deformities in the lumen.
Conclusion Peritumoral electroacupuncture may induce microvasculature normalization by decreasing microvascular density and increasing pericyte coverage of the neovasculature, thereby improving hypoxic microenvironment of breast cancer in mice.
- Electroacupuncture,
- Breast cancer,
- Microvasculature normalization,
- Endothelial cells,
- Pericytes

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Preliminary Study on Microvasculature Normalization Induced by Peritumoral Electroacupuncture in Mice With Breast Cancer Xenografts

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