Myrislignan Induces Apoptosis in Gastric Cancer Cell Line Through PI3K/AKT Signaling Pathway

ZHOU Yong-jun; PAN Yong-yue; YANG Li-jun; BIE Ming-jiang

doi:10.12182/20230160101

Volume 54 Issue 1

Jan. 2023

Turn off MathJax

Article Contents

Abstract

References

JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) > 2023 > 54(1): 136-141. > DOI: 10.12182/20230160101

ZHOU Yong-jun, PAN Yong-yue, YANG Li-jun, et al. Myrislignan Induces Apoptosis in Gastric Cancer Cell Line Through PI3K/AKT Signaling Pathway[J]. Journal of Sichuan University (Medical Sciences), 2023, 54(1): 136-141. DOI: 10.12182/20230160101

Citation:

PDF (1340 KB)

Myrislignan Induces Apoptosis in Gastric Cancer Cell Line Through PI3K/AKT Signaling Pathway

1.
Chengdu Lezhu Biotechnology Ltd., Chengdu 610041, China
2.
Tibet University, Lhasa 850000, China
3.
Department of Clinical Laboratory, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China
4.
Editorial Board of Journal of Sichuan University (Medical Sciences), Chengdu 610041, China

More Information

Corresponding author:
BIE Ming-jiang, E-mail: 13941057@qq.com
Received Date: March 27, 2022
Revised Date: September 30, 2022
Available Online: January 16, 2023
Published Date: January 19, 2023

Abstract

Abstract

Objective To investigate the effect of myrislignan (MYR) on the apoptosis of gastric cancer cell line and its relationship with phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway.
Methods The gastric cells (SGC-7901) were treated with MYR at different concentrations, i.e., 0, 25, 50, 100, and 200 μmol/L, for 48 h and 72 h and the effect of MYR on the proliferation of SGC-7901 cells was measured by CCK-8 assay. Then, SGC-7901 cells were treated with different concentrations of MYR at 50, 100, and 200 μmol/L for 48 h. Meanwhile, a normal control group and a dimethyl sulfoxide (DMSO) solvent control group (0.1% DMSO) were established. Flow cytometry was used to determine the apoptosis rate of SGC-7901 cells. The protein expression levels of PI3K, AKT, Bcl-2-associated X protein (BAX), cysteine-dependent aspartate-specifc protease-3 (Caspase-3), and Caspase-9 were determined by Western blot. Then, PI3K activator (20 μmol/mL) was used to treat SGC-7901 cells for 48 h in 4 groups, the control group, 0.1% DMSO group, MYR group, and MYR+PI3K activator group, and the effect on MYR’s induction of apoptosis and regulation of the protein expression levels of PI3K, AKT, BAX, Caspase-3, and Caspase-9 in SGC-7901 cells.
Results Compared with the control group, MYR at 50, 100 and 200 μmol/L inhibited the proliferation of gastric cancer cells, increased the apoptosis rate, down-regulated the protein expression levels of PI3K and AKT, and up-regulated the protein expression levels of BAX, Caspase-3, and Caspase-9 in a dose-dependent manner (P<0.05). However, PI3K activator attenuated MYR-induced apoptosis in gastric cancer cells and MYR’s regulation of PI3K, AKT, BAX, Caspase-3, and Caspase-9 protein expression (P<0.05).
Conclusion MYR induces the expression of BAX, Caspase-3, and Caspase-9 proteins by inhibiting the PI3K/AKT signaling pathway, thereby promoting the apoptosis of gastric cancer cells.
- Myrislignan,
- PI3K/AKT signaling pathway,
- Stomach neoplasms,
- Apoptosis

FullText(HTML)

References (21)

References

[1]	SMYTH E C, NILSSON M, GRABSCH H I, et al. Gastric cancer. Lancet,2020,396(10251): 635–648. DOI: 10.1016/S0140-6736(20)31288-5
[2]	JOSHI S S, BADGWELL B D. Current treatment and recent progress in gastric cancer. CA Cancer J Clin,2021,71(3): 264–279. DOI: 10.3322/caac.21657
[3]	ALSHEHRI A, ALANEZI H, KIM B S. Prognosis factors of advanced gastric cancer according to sex and age. World J Clin Cases,2020,8(9): 1608–1619. DOI: 10.12998/wjcc.v8.i9.1608
[4]	SEENEEVSSEN L, BESSEDE E, MEGRAUD F, et al. Gastric Cancer: Advancesin Carcinogenesis Research and New Therapeutic Strategies. Int J Mol Sci,2021,22(7): 3418–3442. DOI: 10.3390/ijms22073418
[5]	HACKER U, HOFFMEISTER A, LORDICK F. Gastric cancer: diagnosis and current treatment strategies. Dtsch Med Wochenschr,2021,146(23): 1533–1537. DOI: 10.1055/a-1169-0440
[6]	SHI C, YANG E J, TAO S, et al. Natural products targeting cancer cell dependency. J Antibiot,2021,74(10): 677–686. DOI: 10.1038/s41429-021-00438-x
[7]	LU X, YANG L, CHEN J, et al. The action and mechanism of myrislignan on A549 cells in vitro and in vivo. J Nat Med,2017,71(1): 76–85. DOI: 10.1007/s11418-016-1029-6
[8]	JIN H, ZHU Z G, YU P J, et al. Myrislignan attenuates lipopolysaccharide-induced inflammation reaction in murine macrophage cells through inhibition of NF-κB signalling pathway activation. Phytother Res,2012,26(9): 1320–1326. DOI: 10.1002/ptr.3707
[9]	ZHANG J, CHEN J, LV K, et al. Myrislignan induces redox imbalance and activates autophagy in Toxoplasma gondii. Front Cell Infect Microbiol,2021,11: 730222. DOI: 10.3389/fcimb.2021.730222
[10]	YANG X N, LIU X M, FANG J H, et al. PPARα mediates the hepatoprotective effects of nutmeg. J Proteome Res,2018,17(5): 1887–1897. DOI: 10.1021/acs.jproteome.7b00901
[11]	ZHANG L, JIA B, VELU P, et al. Corilagin induces apoptosis and inhibits HMBG1/PI3K/AKT signaling pathways in a rat model of gastric carcinogenesis induced by methylnitronitrosoguanidine. Environ Toxicol,2022,37(5): 1222–1230. DOI: 10.1002/tox.23478
[12]	TAN W, LU J, HUANG M, et al. Anti-cancer natural products isolated from chinese medicinal herbs. Chin Med,2011,6(1): 27–36. DOI: 10.1186/1749-8546-6-27
[13]	CARNEIRO B A, El-DEIRY W S. Targeting apoptosis in cancer therapy. Nat Rev Clin Oncol,2020,17(7): 395–417. DOI: 10.1038/s41571-020-0341-y
[14]	LIU R, CHEN Y, LIU G, et al. PI3K/AKT pathway as a key link modulates the multidrug resistance of cancers. Cell Death Dis,2020,11(9): 797–808. DOI: 10.1038/s41419-020-02998-6
[15]	方正月, 杨凯, 赵丹, 等. LncRNA CASC9高表达激活PI3K/AKT信号通路促进口腔鳞癌细胞增殖转移. 重庆医科大学学报,2019,44(7): 898–904. DOI: 10.13406/j.cnki.cyxb.002169
[16]	姚红兵, 肖芳, 郭威, 等. FAK抑制剂对肝癌HCC-LM3细胞骨架重排和侵袭的作用机制. 中山大学学报(医学科学版),2021,42(3): 364–372. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0106
[17]	JIA X, WEN Z, SUN Q, et al. Apatinib suppresses the proliferation and apoptosis of gastric cancer cells via the PI3K/Akt signaling pathway. J BUON,2019,24(5): 1985–1991.
[18]	SONG M, BODE A M, DONG Z, et al. AKT as a therapeutic target for cancer. Cancer Res,2019,9(6): 1019–1031. DOI: 10.1158/0008-5472.CAN-18-2738
[19]	CHEN W, ZHANG Y, GU X, et al. Qi Ling decoction reduces gastric cancer cell metastasis by inhibiting MMP-9 through the PI3K/Akt signaling pathway. Am J Transl Res,2021,13(5): 4591–4602.
[20]	YU J, SONG S, JIAO J, et al. ZiYinHuaTan recipe inhibits cell proliferation and promotes apoptosis in gastric cancer by suppressing PI3K/AKT pathway. Biomed Res Int,2020,2020: 2018162. DOI: 10.1155/2020/2018162
[21]	ZHOU D, LIU W, LIANG S, et al. Apoptin-derived peptide reverses cisplatin resistance in gastric cancer through the PI3K-AKT signaling pathway. Cancer Med,2018,7(4): 1369–1383. DOI: 10.1002/cam4.1380

OPEN ACCESS This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (CC BY-NC 4.0). In other words, the full-text content of the journal is made freely available for third-party users to copy and redistribute in any medium or format, and to remix, transform, and build upon the content of the journal. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may not use the content of the journal for commercial purposes. For more information about the license, visit https://creativecommons.org/licenses/by-nc/4.0

Relative Articles

Supplements (0)

Cited By

Get Citation

PDF

XML

Article views (833) PDF downloads (38)

Myrislignan Induces Apoptosis in Gastric Cancer Cell Line Through PI3K/AKT Signaling Pathway

Abstract

References

Catalog

Export File

Citation

Format

Content