Welcome to JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) June 11, 2025
Advanced Search
Volume 53 Issue 3
May  2022
Turn off MathJax
Article Contents
Abstract
References
Related Articles
WANG Jie, LIU Jian, WEN Jian-ting, et al. Correlation between circRNA0003353 in Peripheral Blood Mononuclear Cells and Immune Inflammation in Rheumatoid Arthritis Patients with Damp Heat Obstruction Syndrome[J]. Journal of Sichuan University (Medical Sciences), 2022, 53(3): 437-443. DOI: 10.12182/20220560106
Citation: WANG Jie, LIU Jian, WEN Jian-ting, et al. Correlation between circRNA0003353 in Peripheral Blood Mononuclear Cells and Immune Inflammation in Rheumatoid Arthritis Patients with Damp Heat Obstruction Syndrome[J]. Journal of Sichuan University (Medical Sciences), 2022, 53(3): 437-443. DOI: 10.12182/20220560106
shu

Correlation between circRNA0003353 in Peripheral Blood Mononuclear Cells and Immune Inflammation in Rheumatoid Arthritis Patients with Damp Heat Obstruction Syndrome

  • Department of Rheumatology and Immunology, First Affiliated Hospital, Anhui University of Chinese Medicine, Hefei 230031, China

More Information
  • Corresponding author:

    LIU Jian, E-mail: liujianahzy@126.com

  • Received Date: May 16, 2021
  • Revised Date: December 07, 2021
  • Available Online: May 24, 2022
  • Published Date: May 19, 2022
    Abstract
  •   Objective  To investigate the expression of circRNA 0003353 in the peripheral blood mononuclear cells (PBMCs) of rheumatoid arthritis (RA) patients with dampness heat obstruction syndrome and to examine its effect on inflammatory response of fibroblast-like synoviocytes (FLS).
      Methods  The PBMCs and serum samples of 55 RA patients with dampness heat obstruction syndrome and 30 healthy volunteers were collected. The expression of circRNA 0003353 and its correlation with clinical indexes were examined. The circRNA 0003353 overexpression plasmid and siRNA were constructed and transfected into RA-FLS cell line. RT-qPCR was used to determine the expression of circRNA 0003353 mRNA. The expressions of interleukin (IL)-4, IL-10 and IL-17 were examined by ELISA. The expressions of Janus kinase 2 (JAK2), p-JAK2, signal transducers and activators of transcription 3 (STAT3) and p-STAT3 were exmained by Western blot. CCK-8 assay was used to assess cell viability. Cell migration was assessed with Transwell migration assay.
      Results  1) Compared with that of the normal group, the expression of circRNA 003353 in the PBMCs of RA patients with damp heat obstruction syndrome was significantly increased (P<0.05). 2) Pearson correlation analysis showed that circRNA 0003353 was positively correlated with erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), receptor activator of nuclear factor-κ B ligand (RANKL) and DAS28, and circRNA 0003353 was negatively correlated with IL-10 (P<0.05). 3) The findings on the association patterns showed that the increase in circRNA 0003353 was significantly correlated with the increase of ESR, IL-17, CRP and immunoglobulin (Ig) G. 4) Logistic regression analysis showed that circRNA 0003353 was a risk factor for RANKL, CRP and ESR. 5) RT-qPCR results showed that the expression of circRNA 003353 mRNA in pcDNA3.1-circRNA 0003353 group was significantly higher than that in pcDNA3.1-NC group (P<0.05), and that the expression of circRNA 003353 mRNA in si-circRNA 0003353 group was significantly lower than that in si-NC group (P<0.05). 6) ELISA and Western blot results showed that, compared with those of pcDNA3.1-NC group, the expression of IL-10 in pcDNA3.1-circRNA 0003353 group significantly decreased, the expression of IL-17 increased, and p-JAK2/JAK2 and p-STAT3/STAT3 ratios significantly increased (P<0.05). Compared with those of si-NC group, the expression of IL-10 in si-circRNA 0003353 group significantly increased, the expression of IL-17 and JAK2 decreased, and p-JAK2/JAK2 and p-STAT3/STAT3 ratios significantly decreased (P<0.05). 7) The results of CCK-8 and Transwell assays showed that the viability and migration of RA-FLS in pcDNA3.1-circRNA 0003353 group were higher than those in pcDNA3.1-NC group (P<0.05). Compared with those of si-NC group, the viability and migration ability of RA-FLS in si-circRNA 0003353 group decreased (P<0.05).
      Conclusion  The expression of circRNA 0003353 is up-regulated in RA patients with damp heat obstruction syndrome, and it is involved in the pathogenesis of RA by activating the JAK2/STAT3 signaling pathway and promoting the inflammatory response.
    • FullText(HTML)
    • References (23)
  • [1]
    姜泉. 国际中医临床实践指南类风湿关节炎(2019-10-11). 世界中医药,2020,15(20): 3160–3168. DOI: 10.3969/j.issn.1673-7202.2020.20.034
    [2]
    VEIGAS B, MATIAS A, CALMEIRO T, et al. Antibody modified gold nanoparticles for fast colorimetric screening of rheumatoid arthritis. Analyst,2019,144(11): 3613–3619. DOI: 10.1039/C9AN00319C
    [3]
    XU Q, YIN S, YAO Y, et al. MAST3 modulates the inflammatory response and proliferation of fibroblast-like synoviocytes in rheumatoid arthritis. Int Immunopharmacol, 2019, 77: 105900[2021-03-18]. https://doi.org/10.1016/j.intimp.2019.105900.
    [4]
    NAQVI A, HASSALI M, NAQVI S, et al. Estimation of direct cost of managing rheumatoid arthritis treatment to Pakistani patients using real-world follow-up data. Int J Rheum Dis,2020,23(3): 325–333. DOI: 10.1111/1756-185X.13776
    [5]
    袁林, 吴金玉, 张之燕, 等. 中医治疗类风湿关节炎风湿热证的研究进展. 湖南中医杂志,2020,36(9): 195–197.
    [6]
    韩善夯. 类风湿关节炎中医证候分型与自身抗体的相关研究. 山东中医杂志,2018,37(7): 584–587.
    [7]
    文建庭, 刘健, 王馨, 等. 新风胶囊含药血清对TNF-α诱导的类风湿关节炎滑膜成纤维细胞凋亡和炎症的影响. 中国中药杂志,2021,46(2): 436–443.
    [8]
    DEL REY M J, VALÍN Á, USATEGUI A, et al. Senescent synovial fibroblasts accumulate prematurely in rheumatoid arthritis tissues and display an enhanced inflammatory phenotype. Immun Ageing, 2019, 16: 29[2021-03-18]. https://immunityageing.biomedcentral.com/articles/10.1186/s12979-019-0169-4. doi: 10.1186/s12979-019-0169-4.
    [9]
    雷波, 玄秀云, 樊卫平. CircRNA在自身免疫疾病中的研究进展. 中国生物制品学杂志,2019,32(3): 347–350.
    [10]
    王杰, 刘健, 文建庭, 等. 环状RNAs与类风湿关节炎骨破坏潜在关系的研究. 海南医学院学报,2021,27(24): 1916–1920. DOI: 10.13210/j.cnki.jhmu.20201012.002
    [11]
    WANG J, YAN S, YANG J, et al. Non-coding RNAs in rheumatoid arthritis: From bench to bedside. Front Immunol, 2020, 10: 3129[2021-03-18]. https://doi.org/10.3389/fimmu.2019.03129.
    [12]
    YANG X, LI J, WU Y, et al. Aberrant dysregulated circular RNAs in the peripheral blood mononuclear cells of patients with rheumatoid arthritis revealed by RNA sequencing: Novel diagnostic markers for RA. Scand J Clin Lab Invest,2019,79(8): 551–559. DOI: 10.1080/00365513.2019.1674004
    [13]
    EL-GHAFAR O A M A, HELAL G K, ABO-YOUSSEF A M. Apixaban exhibits anti-arthritic effects by inhibiting activated factor X-mediated JAK2/STAT3 and MAPK phosphorylation pathways. Inflammopharmacology,2020,28(5): 1253–1267. DOI: 10.1007/s10787-020-00693-8
    [14]
    孙艳秋. 健脾化湿通络法治疗类风湿关节炎贫血患者的数据挖掘及对JAK2/STAT3信号通路的影响. 合肥: 安徽中医药大学, 2020.
    [15]
    苗正月, 蒋金桃, 崔国良, 等. 类风湿关节炎中相关促炎因子和抑炎因子的研究进展. 医学综述,2021,27(18): 3569–3573. DOI: 10.3969/j.issn.1006-2084.2021.18.007
    [16]
    HECKERT S L, BERGSTRA S A, MATTHIJSSEN X M E, et al. Joint inflammation tends to recur in the same joints during the rheumatoid arthritis disease course. Ann Rheum Dis,2022,81(2): 169–174. DOI: 10.1136/annrheumdis-2021-220882
    [17]
    WEN J, LIU J, WANG X, et al. Expression and clinical significance of circular RNAs related to immunity and inflammation in patients with rheumatoid arthritis. Int Immunopharmacol, 2021, 92: 107366[2021-03-18]. https://doi.org/10.1016/j.intimp.2021.107366.
    [18]
    孙艳秋, 刘健, 忻凌, 等. 黄芩清热除痹胶囊联合新风胶囊改善类风湿关节炎湿热证患者免疫炎症指标的数据挖掘研究. 风湿病与关节炎,2020,9(1): 5–9. DOI: 10.3969/j.issn.2095-4174.2020.01.002
    [19]
    OUYANG Q, WU J, JIANG Z, et al. Microarray expression profile of circular RNAs in peripheral blood mononuclear cells from rheumatoid arthritis patients. Cell Physiol Biochem,2017,42(2): 651–659. DOI: 10.1159/000477883
    [20]
    ZHONG S, OUYANG Q, ZHU D, et al. Hsa_circ_0088036 promotes the proliferation and migration of fibroblast-like synoviocytes by sponging miR-140-3p and upregulating SIRT 1 expression in rheumatoid arthritis. Mol Immunol,2020,125: 131–139. DOI: 10.1016/j.molimm.2020.07.004
    [21]
    CAI Y, LIANG R, XIAO S, et al. Circ_0088194 promotes the invasion and migration of rheumatoid arthritis fibroblast-like synoviocytes via the miR-766-3p/MMP2 axis. Front Immunol, 2021, 12: 628654[2021-11-10]. https://doi.org/10.3389/fimmu.2021.628654.
    [22]
    YANG J, CHENG M, GU B, et al. CircRNA_09505 aggravates inflammation and joint damage in collagen-induced arthritis mice via miR-6089/AKT1/NF-κB axis. Cell Death Dis,2020,11(10): 833. DOI: 10.1038/s41419-020-03038-z
    [23]
    LIU W, SONG J, FENG X, et al. LncRNA XIST is involved in rheumatoid arthritis fibroblast-like synoviocytes by sponging miR-126-3p via the NF-κB pathway. Autoimmunity,2021,54(6): 326–335. DOI: 10.1080/08916934.2021.1937608
    • Relative Articles

  • Related Articles

    [1]GAO Huihui, QIAN Beiran, NI Yan, SUN Liying, FU Junfen. Research Progress in the Pathogenesis of Polycystic Ovary Syndrome[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2024, 55(4): 1049-1054. DOI: 10.12182/20240760208
    [2]ZHAO Yinzhen, LI Yulin, LI Jiao, NI Mingli, WANG Jichuang, WANG Xiaojun, CHENG Lei, NIU Wenge, ZHANG Yingfu, WANG Yunlong. Establishment of an Engineered Bacterial Membrane Biomimetic Nanodrug Delivery System and Its Role in the Treatment of Glioma[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2024, 55(4): 861-871. DOI: 10.12182/20240760203
    [3]XIE Lixin, DU Zhefei, PENG Qiuxia, ZHANG Kun, FANG Chao. Classification and Application of Ultrasound-Responsive Nanomaterials in Anti-Inflammatory Therapy[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2024, 55(4): 793-799. DOI: 10.12182/20240760104
    [4]YONG Sheng, GUO Yujing, CHEN Xiaochen, XU Yuzhen, HU Ying. Mechanism of IL-17 Signaling Pathway in Spleen Inflammatory Response Induced by Altitude Hypoxia in Mice[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2024, 55(1): 118-124. DOI: 10.12182/20240160208
    [5]WANG Xin-yan, DU Xin-mei, ZHOU Xue-dong. New Developments in Research on the Relationship Between Osteoarthritis and Oral-Gut Microbes[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2023, 54(1): 49-53. DOI: 10.12182/20230160508
    [6]YU Hong-biao, SHI Meng-dan, YAO Jing, JIA Jin, ZHU Cai-rong, ZHOU Rong. Value of Neutrophil-to-Lymphocyte Ratio and Platelet-to-Lymphocyte Ratio for Predicting Preeclampsia During Pregnancy[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2022, 53(6): 1039-1044. DOI: 10.12182/20221160202
    [7]JI Li-wei, DENG Yan, LI Tao. Effect of Ketone Body β-Hydroxybutyrate to Attenuate Inflammation-Induced Mitochondrial Oxidative Stress in Vascular Endothelial Cells[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2021, 52(6): 954-959. DOI: 10.12182/20211160202
    [8]SUN Ya-jun, ZHANG Yan-fang, XU Hui-min, MA Yong-tao, LI Chun, NIE Chun-hong, ZHAO Min. Morin Improves Experimental Autoimmune Thyroiditis in Rats via NLRP3/Caspase-1 Pathway[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2021, 52(2): 229-234. DOI: 10.12182/20210160507
    [9]QIN Qiu-yu, LÜ Xiang-wei, WU Qi, LU Pan, ZHAO Wei-kun. 6-Gingerol Pretreatment Alleviates Hypoxia/Reoxygenation-induced H9C2 Cardiomyocyte Injury by Inhibiting Oxidative Stress and Inflammation[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2020, 51(5): 658-663. DOI: 10.12182/20200960606
    [10]WANG Lu, ZHANG Li-ning, HE Jia-le, ZUO Xiu-qin, XIE Hui-min, JIA Zi-shan. The Therapeutic Effect and Mechanism of Static Progressive Stretching in Different Durations on Traumatic Knee Contracture in Rats[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2020, 51(2): 185-192. DOI: 10.12182/20200360204
    • Supplements (0)

  • Cited by

    Periodical cited type(6)

    1. 许炬珍，张丽卿. JAK/STAT信号通路在类风湿关节炎致病机制及治疗靶点中的作用进展. 河北医药. 2024(04): 601-605+611 .
    2. 冉彩霞，华贤章. 不同中医证型腰椎间盘突出症患者CT表现及腰臀部压敏点分析. 四川中医. 2024(01): 87-90 .
    3. 李文君，杜会娟，李蔷. 艾灸阿是穴配合中药熏蒸对类风湿关节炎患者类风湿因子、炎症因子的影响. 航空航天医学杂志. 2024(07): 840-843 .
    4. 陈焕，金丹，高姗. 类风湿性关节炎滑膜病变高频超声表现与Anti-ccp、ESR及CRP的相关性. 分子诊断与治疗杂志. 2024(10): 1823-1826+1831 .
    5. 刘翠，杜小正，刘莉梅. 外泌体中非编码RNA在类风湿关节炎中作用的研究进展. 中国病理生理杂志. 2023(02): 359-365 .
    6. 朱江波，姜侠，张丽. 咖啡摄入与常见自身免疫性疾病关联性研究进展. 现代预防医学. 2023(14): 2542-2546 .

    Other cited types(2)

Catalog

    Get Citation
    PDF
    XML
    Article views (524) PDF downloads (38) Cited by(8)
    • Website Copyright © Editorial Office of Journal of Sichuan University (Medical Sciences).
    • 17, Section 3, Renmin Nanlu Road, Wuhou District, Chengdu 610041, People’s Republic of China
    • Tel：+86-028-85501320 +86-028-85500106       E-mail: scuxbyxb@scu.edu.cn

    Official website of Journal of Sichuan University (Medical Sciences)

    WeChat official account of Journal of Sichuan University(Medical Sciences)

    WeChat subscription account of Journal of Sichuan University(Medical Sciences)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return