A Preliminary Study on the Effect of CD47-Targeted Immunotherapy on Oral-Gut Microbiota

  Objective  To study the effects of CD47-targted immunotherapy on the oral-gut microbiota of immune-competent mice.

  Methods  A peritoneal metastatic colon cancer model was constructed in immune-competent mice. Anti-CD47 monoclonal antibody was intraperitoneally administered to the mice in the treatment group, while PBS was administered to mice in the control group. Tumor growth was documented with small animal live imaging technology. 16S rRNA sequencing technology was used to analyze the composition and diversity of oral-gut microbiota.

  Results  The alpha diversity of oral microbes in the anti-CD47 monoclonal antibody treatment group decreased, and the difference was statistically significant. There was no significant change in the alpha diversity of gut microbes. Differential species analysis showed significantly decreased abundance of Staphylococcus, Jeotgalicoccus, and Sporosarcina in the oral microbiota of mice in the treatment group compared to that of mice in the control group. The abundance of Bacteroides in the gut microbiota was significantly higher in the treatment group.

  Conclusion  CD47-targted immunotherapy has a rather significant impact on the diversity of oral microbiota in mice, but does not have significant impact on the species diversity of gut microbiota.