Preparation and Release Properties of <i>Helicobacter pylori</i> Recombinant Protein PLGA Microspheres and PLGA-Chitosan Microspheres

CHEN Yu-zuo; LIU Ren-jie; YANG Lu; LI Mao-yuan; LI Wan-yi; WANG Bao-ning

doi:10.12182/20210960208

Volume 52 Issue 5

Sep. 2021

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JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) > 2021 > 52(5): 794-798. > DOI: 10.12182/20210960208

CHEN Yu-zuo, LIU Ren-jie, YANG Lu, et al. Preparation and Release Properties of Helicobacter pylori Recombinant Protein PLGA Microspheres and PLGA-Chitosan Microspheres[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(5): 794-798. DOI: 10.12182/20210960208

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Preparation and Release Properties of Helicobacter pylori Recombinant Protein PLGA Microspheres and PLGA-Chitosan Microspheres

1.
Department of Pathogenic Biology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China
2.
Chengdu Blood Center, Chengdu 610041, China
3.
Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China

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Corresponding author:
WANG Bao-ning, E-mail: 345182273@qq.com
Received Date: October 11, 2020
Revised Date: September 01, 2021
Available Online: September 21, 2021
Published Date: September 19, 2021

Abstract

Abstract

Objective To preparethe poly lactic-co-glycolic acid (PLGA) microspheres and PLGA-chitosan microspheres containing Helicobacter pylori recombinant protein, namely the BIB protein, and to explore their optimal preparation parameters and in vitro release performance in gastric and intestinal fluids.
Methods Double emulsions (water-in-oil-in-water, or W1/O/W2) solvent evaporation method was used to prepare the BIB-PLGA microspheres and the BIB-PLGA-chitosan microspheres. Univariate analysis was done to study the impact of the water-to-oil ratio (W1/O), PLGA mass fraction and PVA concentration on the morphology, particle size, polydispersity index (PDI), encapsulation efficiency (EE), and drug loading (DL) so as to identify the optimal parameters. Bicinchoninic acid (BCA) assay was used to determine the protein concentration and the release efficiency of BIB.
Results The optimal preparation parameters identified in the study were as follows: W1/O at 1∶2, PLGA mass fraction at 5%, and PVA mass fraction at 0.2%. The BIB-PLGA microspheres were found to be (2.11±0.08) μm in particle size, 0.35±0.18 in PDI, (78.20±1.73)% in EE and (10.58±0.23)% in DL. The BIB-PLGA-chitosan microspheres were (2.28±0.52) μm in particle size, 0.39±0.54 in PDI, and (78.87±1.30)% and (15.50±0.25)% in EE and DL, respectively. Both BIB-PLGA microspheres and BIB-PLGA-chitosan microspheres showed slow-release property in gastric and intestinal fluids in vitro, with BIB-PLGA-chitosan microspheres showing better slow-release performance.
Conclusion The BIB-PLGA microspheres and BIB-PLGA-chitosan microspheres prepared with the double emulsions solvent evaporation method showed high DL and EE, controllable particle sizes, dispersive appearance, and slow-release property in gastric and intestinal fluids in vitro.

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References

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