Influence of Dingkun Dan Combined with Estradiol Valerateon Wnt/β-catenin Signaling Pathway in Rats with Thin Endometrium with Kidney-Yang Deficiency

ZHENG Juan; TAN Yong

doi:10.12182/20210160508

Volume 52 Issue 2

Mar. 2021

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JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) > 2021 > 52(2): 235-240. > DOI: 10.12182/20210160508

ZHENG Juan, TAN Yong. Influence of Dingkun Dan Combined with Estradiol Valerateon Wnt/β-catenin Signaling Pathway in Rats with Thin Endometrium with Kidney-Yang Deficiency[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(2): 235-240. DOI: 10.12182/20210160508

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Influence of Dingkun Dan Combined with Estradiol Valerateon Wnt/β-catenin Signaling Pathway in Rats with Thin Endometrium with Kidney-Yang Deficiency

ZHENG Juan^{1, 2,},
TAN Yong^2, ,

1.
Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou 450002, China
2.
Nanjing University of Chinese Medicine, Nanjing 210046, China

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Corresponding author:
TAN Yong, E-mail: 1516584093@qq.com
Received Date: December 18, 2019
Revised Date: October 17, 2020
Available Online: March 21, 2021
Published Date: March 19, 2021

Abstract

Abstract

Objective To explore the influence for combination of Dingkun Dan with estradiol valerateon in treating rats with thin endometrium with Kidney-Yang deficiency based on Wnt/β-catenin signaling pathway.
Methods The estrous period 40 rats were randomly divided into the normal control group, the model group, the Dingkun Dan group, the estradiol valerateon group and the combination group, with 8 rats in each group. In addition to the normal control group, the rat model of thin endometrium with Kidney-Yang deficiency was established in other groups. The control group used free diet, the model group was given distilled water, the estradiol valerateon group was treated with progynova by gavage at 0.3 mg/(kg·d) , Dingkun Dan group was treated with Dingkun Dan by gavage at 2.26 g/(kg·d), and the combined group was given Dingkun Dan at 2.26 g/(kg·d) on the basis of progynova at 0.3 mg/(kg·d). After 3 estrous cycles, the rats were killed and harvested. HE staining was used to observe histopathologic changes in endometrium. The expression of VEGF in rats endometrium were detected by immunohistochemistry. The expression of β-catenin, E-cadherin and MMP-9 protein in rat endometrium was detected by Western blot.
Results Compared with the control group, the uterine cavity was narrowed or enlarged, the endometrium glands and blood vessels were sparse, and the endometrium was thinner significantly in the model group (P<0.01); the levels of VEGF was decreased significantly (P<0.01), while β-catenin, E-cadherin and MMP-9 were increased significantly (P<0.01). Compared with the model group, more endometrial glands, rich intimal vessels, the endometrium were thickened significantly in the 3 treatment groups (P<0.01 or P<0.05); the levels of VEGF was increased differently. The protein levels of β-catenin and E-cadherin were significantly decreased in each treatment group (P<0.01), and MMP-9 were significantly decreased in the Dingkun Dan group and in the combination group (P<0.01). Compared with the estradiol valerateon group, the level of β-catenin in Dingkun Dan group was higher, and MMP-9 was lower (P<0.05 or P<0.01). The levels of β-catenin and MMP-9 in the combination group were significantly decreased (P<0.01). Compared with the combination group, the levels of β-catenin was increased significantly, while decreased signicantly in Dingkun Dan group (P<0.01 or P<0.05).
Conclusion Dingkun Dan combined with estradiol valerateon can increase the thicken of the endometrium by up-regulation of VEGF, while down-regulate of β-catenin, E-cadherin and MMP-9 in rats with Shen-Yang deficiency and thin endometrium.
- Dingkun Dan,
- Thin endometrium,
- VEGF,
- β-catenin

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Influence of Dingkun Dan Combined with Estradiol Valerateon Wnt/β-catenin Signaling Pathway in Rats with Thin Endometrium with Kidney-Yang Deficiency

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