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Volume 51 Issue 6
Nov.  2020
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GONG Tao, CUI Yu-jia, ZHOU Xue-dong, et al. Synergistic Antibacterial Activity of Berberine in Combination with Amylmetacresol Against Enterococcus faecalis in vitro[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(6): 749-754. DOI: 10.12182/20201160501
Citation: GONG Tao, CUI Yu-jia, ZHOU Xue-dong, et al. Synergistic Antibacterial Activity of Berberine in Combination with Amylmetacresol Against Enterococcus faecalis in vitro[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(6): 749-754. DOI: 10.12182/20201160501
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Synergistic Antibacterial Activity of Berberine in Combination with Amylmetacresol Against Enterococcus faecalis in vitro

  • State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

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  • Received Date: June 19, 2020
  • Revised Date: September 08, 2020
  • Available Online: November 19, 2020
  • Published Date: November 19, 2020
    Abstract
  •   Objective  To study the antibacterial effect of berberine combined with amylmetacresol on Enterococcus faecalis.
      Methods  Both dilution method and live bacteria CFU were used to determine the minimum inhibitory concentration (MIC) of berberine and amylmetacresol on E. faecalis. The killing effect of berberine and amylmetacresol on planktonic E. faecalis was detected by suspension quantitative germicidal test and live/dead bacteria staining. The effects of berberine and amylmetacresol on the structure of mature biofilm of E. faecalis was observed by scanning electron microscopy (SEM). The toxicity of berberine and amylmetacresol on human oral keratinocytes (HOK) was determined by CCK-8 cell proliferation and cytotoxicity assay and cytotoxicity LDH assay.
      Results  The MIC of berberine was 512 μg/mL, and the MIC of amylmetacresol was 0.023 3%. 512 μg/mL berberine and 0.002 33% amylmetacresol had a weak killing effect on planktonic E. faecalis alone, while they showed a synergistic antibacterial effect in combination. Cell survival in the biofilm was only slightly changed by berberine and amylmetacresol. The structure of biofilm was obviously changed by berberine and amylmetacresol. 512 μg/mL berberine and 0.002 33% amylmetacresol alone or in combination showed the survival rate was much higher than the injury rate, suggesting berberine and amylmetacresol had a low cytotoxicity.
      Conclusion  Berberine and amylmetacresol had synergism against E. faecalis, and the biological safety of the combination use was better.
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