Welcome to JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES) June 11, 2025
Advanced Search
Volume 51 Issue 6
Nov.  2020
Turn off MathJax
Article Contents
Abstract
References
Related Articles
GUO Qin, GAO Rui-ping, TAO Ying. Expression of PGC-1ɑ in Ovarian Tissue of PCOS Rat Model[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(6): 817-821. DOI: 10.12182/20200960603
Citation: GUO Qin, GAO Rui-ping, TAO Ying. Expression of PGC-1ɑ in Ovarian Tissue of PCOS Rat Model[J]. Journal of Sichuan University (Medical Sciences), 2020, 51(6): 817-821. DOI: 10.12182/20200960603
shu

Expression of PGC-1ɑ in Ovarian Tissue of PCOS Rat Model

  • Department of Gynaecology and Obstetrics, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China

Funds: the Medical Science and Technology Research Funding of Guangdong Province (A2019364)
More Information
  • Corresponding author:

    Ying Tao; 1140832540@qq.com

  • Received Date: October 07, 2019
  • Revised Date: March 11, 2020
  • Available Online: November 19, 2020
  • Published Date: November 19, 2020
    Abstract
  •   Objective  To investigate the expression of peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α) in the ovarian tissue of polycystic ovary syndrome (PCOS) rat model and obese PCOS rat model, and the possible mechanism of PCOS.
      Methods  Thirty SD rats were randomly divided into control group, PCOS rat model group and obese PCOS rat model group. DHEA dissolved in 0.2 mL soybean oil was injected daily into the rats of two PCOS groups for 21 d. Rats in obese PCOS model group were added with high-fat diet based on DHEA modeling, and each group had 10 rats. Body mass were measured before and on the 22nd day after modeling. The serum testosterone (T) levels were measured by abdominal aortic blood, and the ovarian tissues of rats were taken for histological changes were observed by HE staining, immunohistochemical staining and Western blot to detect PGC-1ɑ protein expression.
      Results  The body mass of rats in each group increased after modeling, and the body mass of rats in PCOS group and obese PCOS group increased significantly (P<0.05). The serum T concentration of two PCOS model groups was higher than that of control group (P<0.01). The serum T concentration in obese PCOS model group was higher than that in the PCOS group (P<0.05). The results of HE staining of rat ovarian tissue showed that there were follicles and a small amount of corpus luteum at different developmental stages in the control group, and the granulosa cells were arranged in 4-6 layers. The number of immature small follicles in the two PCOS groups was significantly increased. The granulosa cells were arranged in 1-3 layers, relatively looser, and some follicles were atresia. In the obese PCOS group, the diameter of ovarian atretic follicles increased, the number of granulocyte layers decreased, and oocytes disappeared more obviously. Immunohistochemical staining showed that the PGC-1ɑ protein was mainly expressed in the cumulus and granulosa cells of ovarian tissue in the control group. The mean gray level of PGC-1ɑ protein expression in PCOS group (0.53±0.06) and obese PCOS group (0.36±0.03) was lower than that of the control group (0.75±0.03), with the statistical difference (P<0.05). PGC-1ɑ expression in the obese PCOS group was lower than that in the PCOS group (P<0.01). The results of Western blot were consistent with those of immunohistochemical staining.
      Conclusion  PGC-1ɑ is associated with damage of ovarian granulosa cells in high-fat environment. The decrease of PGC-1ɑ expression in granulosa cells of ovarian follicles may be an important cause of PCOS development.
    • FullText(HTML)
    • References (15)
  • [1]
    PRATHAP A, SUBHALAKSHMI T P, VARGHESE P J. A cross-sectional study on the proportion of anxiety and depression and determinants of quality of life in polycystic ovarian disease. Indian J Psychol Med,2018,40(3): 257–262. DOI: 10.4103/IJPSYM.IJPSYM_221_17
    [2]
    Rotterdam ESHRE/ASRM-sponsored PCOS consensus workshop group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril,2004,81(1): 19–25. DOI: 10.1016/j.fertnstert.2003.10.004
    [3]
    BILAL M, HASEEB A, REHMAN A. Relationship of polycystic ovarian syndrome with cardiovascular risk factors. Diabetes Metab Syndr,2018,12(3): 375–380. DOI: 10.1016/j.dsx.2018.01.006
    [4]
    CALDWELL A S, MIDDLETON L J, JIMENEZ M, et al. Characterization of reproductive, metabolic, and endocrine features of polycystic ovary syndrome in female hyperandrogenic mouse models. Endocrinology,2014,155(8): 3146–3159. DOI: 10.1210/en.2014-1196
    [5]
    SANTOSO S, WIHADMADYATAMI H, BAKCHOUL T, et al. Role of PGC-1ɑ in vascular regulation: implications for atherosclerosis. Arterioscler Thromb Vasc Biol,2016,36(8): 1467–1473. DOI: 10.1161/ATVBAHA.116.307123
    [6]
    ST-PIERRE J, DRORI S, UIDRY M, et al. Suppression of reactive oxygen species and neurodegeneration by the PGC-1 transcriptional coactivators. Cell,2016,127(2): 397–408. DOI: 10.1016/j.cell.2006.09.024
    [7]
    张晓薇, 邝健全, 曾爱群, 等. 脱氢表雄酮诱导多囊卵巢综合征动物模型的研究. 广州医学院学报,2000,28(3): 14–17.
    [8]
    ZHANG X, ZHANG C, SHEN S, et al. Dehydroepiandrosterone induces ovarian and uterine hyperfibrosis in female rats. Hum Reprod,2013,28(11): 3074–3085. DOI: 10.1093/humrep/det341
    [9]
    ZHENG Q, LI Y, ZHANG D, et al. ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats. Cell Death Dis, 2017, 8(10): e3145[2019-10-26].https://doi.org/10.1038/cddis.2017.494.
    [10]
    FOGL C, PUCKEY L, HINSSEN U, et al. A structural and functional dissection of the cardiac stress response factor MS1. Proteins,2012,80(2): 398–409. DOI: 10.1002/prot.23201
    [11]
    WU S, DIVALL S, NWAOPARA A, et al. Obesity-induced infertility and hyperandrogenism are corrected by deletion of theinsulin receptor in the ovarian theca cell. Diabetes,2014,63(4): 1270–1282. DOI: 10.2337/db13-1514
    [12]
    HAEMMERLE G, MOUSTAFA T, WOELKART G, et al. ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR-ɑ and PGC-1ɑ. Nat Med,2011,17(9): 1076–1085. DOI: 10.1038/nm.2439
    [13]
    YAZAWA T, INAOKA Y, OKADA R, et al. PPAR-gamma coactivator-1 alpha regulates progesterone production in ovarian granulosa cells with SF-l and LRH-l. Mol Endocrinol,2010,24(3): 485–496. DOI: 10.1210/me.2009-0352
    [14]
    LIN J, WU P H, TARR P T, et al. Defects in adaptive energy metabolism with CNS-linked hyperactivity in PGC-1alpha null mice. Cell,2004,119(1): 121–135.
    [15]
    ESPINOZA D O, BOROS L G, CRUNKHORN S, et al. Dual modulation of both lipid oxidation and synthesis by peroxisome proliferator-activated receptor coactivator-1α and -1β in cultured myotubes. FASEB J,2010,24(4): 1003–1014. DOI: 10.1096/fj.09-133728
    • Relative Articles

  • Related Articles

    [1]LI Hui, ZHANG Lihan, HUANG Rongshuang, REN Qian, GUO Fan, SHI Min, YANG Letian, YU Yang, MA Liang, FU Ping. Sichuan Dark Tea-Based Medicated Dietary Formula Improves Obesity-Induced Renal Lipid Metabolism Disorder in Mice by Remodeling Gut Microbiota and Short-Chain Fatty Acid Metabolism[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2023, 54(6): 1112-1120. DOI: 10.12182/20231160208
    [2]ZHANG Wei, MA Xiao, CHENG Hao, ZHANG Dun-fang. Research Progress in High-Sugar Diet and Inflammatory Diseases[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2022, 53(3): 538-542. DOI: 10.12182/20220560103
    [3]DENG Si-si, CHEN Jing, LIU Xue, DONG Xin-yan, ZHANG Xiang-ling, WANG Guo-qing. Protective Effects of Akkermansia muciniphila and Amuc_1100 Protein on Rats on High-Fat Diet Combined with Streptozotocin Injection[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2022, 53(1): 83-91. DOI: 10.12182/20220160304
    [4]WANG Sen-jia, ZHENG Xiang, BI Wen-jie, ZHOU Xue. Obesity Combined with Chronic Restraint Stress-Induced Hypertension in Mice Is Associated with the Damage of Noradrenergic Neurons in Nucleus Tractus Solitarii[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2022, 53(1): 77-82. DOI: 10.12182/20220160505
    [5]CHEN Ni, HAN Pengding, CHEN Wen. et al. Investigation the Inhibitory Effects of Kaempferol on Rat Renalmesangial Cells Proliferation under High [J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2017, 48(4): 526-530.
    [6]脊髓血管网织细胞瘤的分型与显微手术治疗
    [J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2016, 47(3): 437-439.
    [7]SUN Xiao-dong, YU Ye-rong. The Protective Effects of Rosiglitazone on Kidney in Diet-induced Obese Rats[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2014, 45(1): 24-28.
    [8]ZHANG Rong-rong, SONG Yong-yan, FAN Mei, LI Yuan-hao, JIANG Zhe, ZHANG Zhen, FANG Ding-zhi. Effects of an Insertion/Deletion Polymorphism of Angiotensin Converting Enzyme Gene on the Changes of Serum Lipid Ratios and Blood Pressure Induced by a High-carbohydrate and Low-fat Diet[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2013, 44(5): 731-735.
    [9]HU Min-shan, LI Zheng-ke, FANG Ding-zhi. The Association Study of the LIPC -250G/A Polymorphism and High-carbohydrate/Low-fat Diet Induced Serum Lipid and Apolipoprotein Concentration Changes in Healthy Youth[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2013, 44(5): 727-730,735.
    [10]LI Kun, LI Feng, GUO Li-juan, LI Jie, HUANG Ke, GUO Wei-hua, TIAN Wei-dong. Increased Survival of Free Fat Grafts with Adipose-derived Stem Cells and Platelet-rich Plasma in Nude Mice[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCES), 2013, 44(3): 337-341.
    • Supplements (0)

  • Cited by

    Periodical cited type(4)

    1. 胡渊. 多囊卵巢综合征病人血清Ⅲ型纤连蛋白域蛋白5、过氧化物酶体增殖物激活受体γ辅助激活因子-1α水平与胰岛素抵抗的关系. 安徽医药. 2024(06): 1231-1234 .
    2. 王新艳，肖成炜，梁琳. PGC-1α、FNDC5蛋白在来曲唑诱导多囊卵巢综合征大鼠卵巢组织中的表达. 山东医药. 2024(19): 49-52 .
    3. 张艺，张继，叶彩云，王瑞华，党璇. 芍药苷对多囊卵巢综合征大鼠卵巢颗粒细胞增殖和凋亡的影响机制. 实用药物与临床. 2023(07): 581-586 .
    4. 王立娜，陈慧平，田文艳，颜琪，张慧英. 线粒体功能障碍与多囊卵巢综合征相关性的研究进展. 天津医药. 2022(12): 1330-1335 .

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views (3809) PDF downloads (150) Cited by(4)
    • Website Copyright © Editorial Office of Journal of Sichuan University (Medical Sciences).
    • 17, Section 3, Renmin Nanlu Road, Wuhou District, Chengdu 610041, People’s Republic of China
    • Tel：+86-028-85501320 +86-028-85500106       E-mail: scuxbyxb@scu.edu.cn

    Official website of Journal of Sichuan University (Medical Sciences)

    WeChat official account of Journal of Sichuan University(Medical Sciences)

    WeChat subscription account of Journal of Sichuan University(Medical Sciences)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return